诱导多能干细胞向角膜上皮细胞分化的研究进展

Advance in the induction of the differentiation of pluripotent stem cells into corneal epithelial cells

诱导多能干细胞定向分化为角膜上皮细胞(CECs)用于角膜缘干细胞缺乏症(LSCD)的治疗,具有广阔的前景。目前,这方面研究已经取得诸多成果,但也存在诱导效率不高或诱导时间过长的问题。本文总结了将多能干细胞诱导分化为角膜上皮细胞的方法及诱导信号分子,分析了角膜上皮样细胞的临床应用前景,为重建眼表提供高增殖能力的种子细胞和最终临床应用提供参考和指导。

The induction of the differentiation of pluripotent stem cells into corneal epithelial cells for the treatment of limbal stem cell deficiency hasbroad prospect. Currently, great achievements have been made in the technique of inducing the pluripotent stem cells into the corneal epithelial cells. However, problems such as low efficiency and long induction time still exist. In order to provide seed cells with high proliferative ability for the reconstruction of ocular surface and provide reference and guidance for clinical application, we summarized the methods of including pluripotent stem cells into the corneal epithelial cells. Additionally, we analyzed the clinical application prospect of pluripotent stem cells differentiate into corneal epithelial cells.