基于病证结合方证对应理论研究肝郁证哮喘模型的尿液代谢组学表征

Study on urine metabolomics for asthma of liver-depression syndrome modle based on theories of combination of disease and syndrome with prescriptions corresponding

目的:采用代谢组学方法研究肝郁证哮喘模型的尿液中代谢物含量的变化。方法:采用慢性束缚加卵蛋白致敏法建立肝郁证哮喘模型。收集大鼠尿液并采用液相色谱质谱联用仪分析,将数据导入SIMCA-P13.0软件进行主成分分析和偏最小二乘判别分析,观察各组大鼠尿液的代谢组分变化。结果:鉴定出14种能够反应肝郁证哮喘模型代谢组学表征的生物标志物,乳酸、柠檬酸等7种代谢物含量较正常组降低,乙酰乙酸、肌酸酐等7种代谢物含量较正常组升高。结论:通过病证结合、以方测证的方式验证了肝郁证哮喘模型的可靠性,并鉴定出能够反映模型代谢特征的生物标志物,为中医治疗肝郁证哮喘的作用机理、作用靶点提供了依据。

Objective：To study the change of urine metabolomics for asthma of liver-depression syndrome modle based on metabolomics method. Methods： Asthma of liver-depression syndrome modle was established by inhaling atomized ovalbumin and chronic restraint. The collected urine was analyzed by LC- MS/MS, and the acquired data were analyzed using principal component analysis （PCA） and partial least squares discriminant analysis （PLS-DA） by SIMCA-PI3.0 software, observed the concentration changes of metabolics in each group. Results： 14 characteristic biomarkers of asthma of liver-depression syndrome modle were identified, the content of lactic acid, citric acid and other 7 kinds of metabolites were lower than those of the normal group, the content of acetoacetic acid, creatinine and other 7 kinds of metabolites were higher than those of the normal group. Conclusion ： The asthma of liver-depression syndrome modle＇ s reliability was verified by taking a square to test certificate and combination of disease and syndrome. The identified biomarkers can reflect the metabolic characteristics of asthma of liver-depression syndrome modle, of which reveal the target and mechanism for treatment of asthma of liver-depression syndrome by Traditional Chinese Medicine.