山慈菇多糖的提取及其抗氧化作用的研究

Extraction and antioxidant activity of polysaccharides from Rhizoma Pleionis

目的提取山慈菇多糖,测定其总糖含量并对其体外抗氧化能力进行初步研究。方法采用热水浸提法提取山慈菇多糖,并通过脱蛋白,醇沉等方法分离纯化山慈菇多糖。采用苯酚-硫酸法测定山慈菇多糖的含量。通过邻苯三酚自氧化法、Fenton法和DPPH法测定山慈菇多糖溶液及对照组VC溶液在浓度分别为0.5mg/mL,1 mg/mL,2 mg/mL,4 mg/mL时对超氧阴离子、DPPH自由基及羟自由基的清除率。结果苯酚-硫酸法测得分离纯化后的山慈菇多糖的浓度为30 mg/mL。山慈菇多糖溶液及VC清除超氧阴离子,DPPH自由基及羟自由基的效率随浓度的增加而增强,两组内差异具有显著性意义(P<0.05,P<0.001)。结论山慈菇多糖在体外具有明显清除超氧阴离子、羟自由基和DPPH自由基的作用,并且在体外的抗氧化作用均随其浓度的增大而增大。

Objective To extract polysaccharides from Rhizoma Pleionis and investigate its antioxidative activity in vitro.Methods The polysaccharides were extracted by hot water from Rhizoma Pleionis and purified by phenol-sulfuric acid colorimetry.The antioxidative effects were determined by autoxidation of pyrogallol method,DPPH free radical scavenging assay and Fenton method.VC was used as a control with the concentration of 0.5,1,2 and 4 mg/mL.Results The content of polysaccharide from Rhizoma Pleionis was 30 mg/mL.It had the function of removing superoxide anion radicals,DPPH free radicals and hydroxyl free radical.The scavenging effect enhanced with the increase of polysaccharide concentration.At the concentration of 0.5 mg/mL,the polysaccharide from Rhizoma Pleionis＇ s capacity to scavenge hydroxyl free radical was higher than VC.Conclusion The polysaccharides from Rhizoma Pleionis could have antioxidative effects by scavenging superoxide anion radicals,DPPH free radicals and hydroxyl free radicals.