大半夏汤对化疗呕吐家兔胃Cajal间质细胞水平影响研究

Effect of Dabanxia Decoction on Gastric Cajal Interstitial Cell Level for Rabbits with Chemotherapy and Vomiting

目的:分析大半夏汤对治疗呕吐家兔胃Cajal间质细胞水平的影响,为揭示大半夏汤止呕机理提供参考依据。方法:选取健康家兔,随机分为对照组、DDP组、大半夏汤高剂量组、大半夏汤中剂量组、大半夏汤低剂量组和胃复安组共6组,对DDP组、大半夏汤高中低剂量组和胃复安组家兔采用腹腔注射顺铂法,制备化疗呕吐家兔,制备成功后,按要求分别给药。连续给药7天后,记录6组家兔24小时内的呕吐物量、呕吐次数等情况;检测各组呕吐家兔胃排空率及血浆SP含量;采用ELISA法,分别检测6组家兔胃Cajal间质细胞(ICC)水平。结果:大半夏汤高中低剂量组、胃复安组家兔的呕吐物量和呕吐次数明显少于DDP组,差异有统计学意义(P<0.05);大半夏汤高中低剂量组、胃复安组胃排空率、SP含量、ICC含量均高于DDP组,差异有统计学意义(P<0.05);大半夏汤高剂量组呕吐物量、呕吐次数、胃排空率、SP含量及ICC含量与胃复安组相近,差异无统计学意义(P>0.05);大半夏汤高剂量组胃排空率、SP含量、ICC含量分别为(68.32±7.19)%、(16.34±2.32)ng/L、(0.26420±0.01621)IU/ml,大半夏汤中剂量组三指标分别为(54.68±6.37)%、(12.50±2.16)ng/L、(0.21325±0.010252)IU/ml,低剂量组三指标分别为(46.15±7.21)%、(9.23±2.08)ng/L、(0.18645±0.005469)IU/ml,三组之间各指标两两比较,差异有统计学意义(P<0.05)。结论:大半夏汤对化疗所致呕吐有较好疗效,能提高化疗呕吐家兔胃ICC含量,大半夏汤的止呕机理可能与其能提升ICC水平有关。

Objective： To analyze the effect of Dabanxia Decoction on gastric cajal interstitial cell level for rabbits with vomiting caused by chemotherapy, and to provide reference for revealing the mechanism of vomiting. Methods ： Healthy rabbits were randomly divided into controlled group, DDP group, high dose group of Dabanxia Decoction, middle dose group of Dabanxia Decoction, low dose group of Dabanxia Decoction and Weifu＇ an group were treated with intraperitoneal injection of cisplatin, the preparation of chemotherapy vomiting rabbits, aftei＂ preparation, according to the requirements were administered. After 7days of continuous administration, the number of vomitons and vomiting in 24rabbits were recorded. The gastric emptying rate and plasma SP content were measured in each group of vomiting rabbits. Six groups of rabbits were detected by ELISA interstitial cells （ICC） levels. Results ： The number of vomiting and vomiting in rabbits were significantly lower than that in the DDP group （ P〈 0. 05 ）. The majority of Dabanxia Decoction high and low dose group, （ P〈0. 05 ）. The rate of vomiting, vomiting, gastric emptying rate, SP content and ICC content in the high dose group were significantly higher than those in the controlled group （P 〈0.05） , and the gastric emptying rate, SP content and ICC content were significantly higher than those in the DDP group （P〈 0. 05）. The gastric emptying rate, SP content and ICC content in the high dose group were （68. 32±7. 19）%, （16. 34±2. 32） ng/ s, respectively, and the difference was not significant （P〈0.05） （12.68±6.37）%, （12.50±2.16） ng/L, （0.21325±0. 010252） IU/ml, and the low dose group were significantly higher than those in the controlled group （P〈0. 05） （46. 15 ± 7. 21 ） %, （9. 23±2. 08） ng/L, （0. 18645±0. 005469） IU/ml, the differences among the three groups were statistically significant （P〈0. 05）. Conclusion ： Dabanxia Decoction has a good effect on chemotherapy-induced vomiting and can improve the content of gastric ICC in rabbits with chemotherapy and vomiting. Most of the diarrhea mechanism may be related to the level of ICC.