孟鲁司特对不同阶段大鼠气管平滑肌细胞Ⅲ型胶原蛋白功能的影响

Effect of Montelukast on the Type Ⅲ Collagen in Rat Airway Smooth Muscle Cells at Different Stages

目的:探讨大鼠哮喘气道重塑过程中不同阶段气管平滑肌细胞(ASMC)分泌的Ⅲ型胶原蛋白功能的变化及孟鲁司特对Ⅲ型胶原蛋白的影响。方法:应用卵蛋白致敏激发建立哮喘气道重塑大鼠模型,原代培养ASMC,通过免疫组织化学方法检测ASMC中Ⅲ型胶原蛋白的表达。结果:哮喘组Ⅲ型胶原蛋白平均灰度值均高于对照组,以卵蛋白激发8周组最高,差异有统计学意义(P均<0.01);孟鲁司特干预组Ⅲ型胶原蛋白平均灰度值明显低于哮喘气道重塑组,尤以孟鲁司特干预2周及4周组差异更显著(P<0.01)。结论:孟鲁司特通过抑制ASMC分泌的Ⅲ型胶原蛋白合成能力而减轻气道重塑。

Objective：To investigate the effect of montelukast on the secretion of type Ⅲ collagen in airway smooth muscle cells（ASMC） during different stages of airway remodeling in asthmatic rats.Methods：Ovalbumin（OVA） was used to establish asthmatic airway remodeling model of SD rats and montelukast was given to interfere.Primary culture purified ASMC,and measured the expression of type Ⅲ collagen in ASMC by immunochemical method.Results：The average gray value of type Ⅲ collagen in asthma group was higher than that of the control group,and OVA motivated 8 weeks group was the highest.The differences were significant（P〈0.01）.The average gray value of type Ⅲ collagen in montelukast group was significantly lower than that in asthmatic airway remodeling group,it was significantly different in 2 weeks group and 4 weeks group（P〈0.01）.Conclusion：Montelukast can reduce airway remodeling by inhibiting ASMC collagen synthesis ability.