子宫内膜癌组织和血清中的CD26表达及其诊断价值

Expression of CD26 in the cancerous tissue and serum of patients with endometrial carcinoma and its diagnostic value

目的:探讨人正常子宫内膜增生期、分泌期和子宫内膜癌(endometrial carcinoma,EC)的病理不同分级组织中的CD26表达及血液中可溶性CD26(soluble CD26,s CD26)表达对EC发生、发展的诊断价值。方法:收集2014年1月至2015年12月南通大学附属医院行诊断性刮宫或行手术治疗患者的子宫内膜组织标本,其中正常子宫内膜增生期和分泌期标本各30例、Ⅰ型EC的G1、G2、G3级组织标本各40例。应用免疫组织化学法检测组织中的CD26表达和验证其表达水平后,采集2016年1月至2017年9月经门诊体检的健康育龄女性和住院治疗Ⅰ型EC患者的血液标本,其中增生期及分泌期各20例、Ⅰ型EC的G1、G2级各20例、G3级15例。ELISA法检测其血清中s CD26表达。结果:免疫组织化学法检测结果显示正常子宫内膜组织中分泌期的CD26表达水平>增生期(P<0.05);Ⅰ型EC的G3级组织中的CD26表达水平>G2级>G1级(P<0.05)。ELISA结果显示s CD26与Ⅰ型EC病理分级呈正相关(P<0.05)。结论:Ⅰ型EC组织及血清中的CD26表达水平与疾病进展呈正相关,血清中s CD26表达可作为EC诊断的标记物。

Objective： To explore the diagnostic value of CD26 expression in endometrial carcinoma （EC） by testing CD26 expression in normal proliferative phase, in secretory phase, and in the tissues of different pathological grades of EC; and by investigating the solu- ble CD26 （sCD26） expression in the serum of non-EC patients and EC patients. Methods： Endometrial tissue specimens were collected from patients who received diagnostic curettage or surgical treatment for other reasons in the Affiliated Hospital of Nantong University from January 2014 to December 2015. The above endometrial specimens, included 30 specimens of normal proliferative phase, 30 specimens of secretory phase, and 120 type I EC tissue samples of G1, G2, and G3 grades. The expression of CD26 among these speci- mens were detected by immunohistochemistry. After the expression level of the CD26 protein was verified at the tissue, we continued to collect blood specimens from the outpatients. These patients received physical examination, and included healthy women of child- bearing age and type I EC patients. The patients received treatment in our hospital from January 2016 to September 2017. Of the b^ood samples collected, 20 cases were in proliferative phase and 20 cases were in secretory phase. Samples were also collected from patients with different grades of EC, including 20 cases of type I EC G1 grades, 20 cases of G2 grades, and 15 cases of G3 grades. The expression level of sCD26 in serum was detected by enzyme-linked immunosorbent assay （ELISA）. Results： According to the results of immunohistochemistry, the order of the CD26 expression levels in normal endometrial tissues were as follows： secretory phase 〉prolif- erative phase, with a statistically significant difference observed among these groups （P〈0.05）. The order of the CD26 expression level among different type 1 EC grades were as follows： G3〉G2〉G1, with statistically significant differences also observed among these groups （P〈0.05）. The results of ELISA suggested that the expression level of CD26 in serum was associated with tumor progression, with statistically significant differences observed among the groups （P〈0.05）. Conclusion： The expression of CD26 in EC tissue and se- rum is associated with disease progression. The expression of CD26 in serum could be used as a marker for the diagnosis of EC.