β-氨基丙腈对SGC-7901细胞增殖、迁移和黏附功能的影响

Effect of lysyl oxidase on invasion and adhesion of gastric cancer cells

目的研究β-氨基丙腈(BAPN)抑制赖氨酰氧化酶(LOX)后,对胃癌细胞SGC-7901体外增殖、迁移和黏附功能的影响。方法培养SGC-7901细胞,分别加入不同浓度的LOX抑制剂BAPN,通过MTT比色法检测对SGC-7901细胞增殖的影响,以同质黏附实验和异质黏附实验检测SGC-7901黏附能力的改变,用体外划痕实验检测SGC-7901迁移能力的变化。结果 0.1 mM、0.2 mM和0.3 mM的BAPN对SGC-7901的增殖无明显影响,0.4 mM和0.8 mM的BAPN抑制SGC-7901的增殖。0.1 mM BAPN对SGC-7901同质黏附、异质黏附和迁移能力无明显作用;0.2 mM和0.3 mM BAPN增强SGC-7901同质黏附,减弱其异质黏附,抑制其体外迁移。结论抑制赖氨酰氧化酶可通过抑制胃癌细胞的异质黏附和迁移能力,增强同质黏附,进而抑制其转移能力。

Objective To study the effect of β -aminopropionitrile （BAPN） inhibiting lysyl oxidase （LOX） on the prolifera- tion,migration and adhesion of the human gastric cancer cell line SGC -7901. Methods SGC -7901 cells were cultured in vitro and exposed in the different concentrations of BAPN,the inhibitor of lysyl oxidase. The cell growth curves were drawn by using the MTT assay. Cell migration was assessed by the Wound - Healing assay. The ability of cell adhesion was detected by using homogeneous adhesion and heterogeneous adhesion experiments. Results The growth of SGC - 7901 cells had not been inhibited after being exposed to 0.1 mM ,0.2 mM ,0.3 mM BAPN. The homogeneity adhesion of SGC -7901 was increased and the heterogeneous adhesion was decreased after being exposed to 0.2 mM and 0.3 mM BAPN. The migration of cells was inhibited after being added 0.2 mM and 0.3 mM BAPN. Conclusion Thedata may suggest that LOX promote the metastasis of cancer cell through enhancing invasion, increasing heterogeneous adhesion and decreasing homogeneity adhesion.