新疆维吾尔族和汉族儿童龋病与人类白细胞抗原-DQB1等位基因多态性的相关性研究

Association between the dental caries and the human leucocyte antigen DQB1 allele polymorphisms among the Uygur and Han children in Xinjiang

目的探讨人类白细胞抗原(HLA)-DQB1等位基因多态性与新疆维吾尔族和汉族儿童龋病的相关性。方法采用聚合酶链式反应-序列特异性引物(PCR-SSP)DNA分型技术对新疆维吾尔族和汉族高龋儿童及健康无龋儿童(n=40)进行HLA-DQB1基因分型,研究HLA-DQB1基因多态性与新疆维吾尔族和汉族儿童龋病的相关性。结果维吾尔族与汉族儿童在HLA-DQB1座位均检出5个特异性基因位点。汉族高龋组患者HLA-DQB1*02等位基因频率(12.5%)显著低于对照组(32.5%),差异有统计学意义(P<0.05,OR=0.297);维吾尔族高龋组患者HLADQB1*05等位基因频率(37.5%)显著高于对照组(17.5%),差异有统计学意义(P<0.05,OR=2.829)。结论新疆维吾尔族和汉族儿童龋病与HLA-DQB1等位基因多态性具有一定的相关性;HLA-DQB1*02基因可能是新疆汉族儿童龋病的保护因子;而HLA-DQB1*05等位基因是新疆维吾尔族儿童龋病的易感基因。

Objective This study aims to investigate the association between human leucocyte antigen （HLA）-DQB1 allele polymorphisms and the presence dental caries among the Uygur and Han children in Xinjiang. Methods HLA-DQB1 allele in the Uygur and Han children with dental caries and healthy control in Xinjiang was tested （n=40） using the polymerase chain reaction-sequence specific primer （PCR-SSP） DNA parting technology. Results A total of five specific loci were detected in the HLA-DQB1 locus among the Uygur and Han children. The frequency of the HLA-DQB1＊02 allele in the Han group with severe caries （12.5%） was significantly lower than in the control group （32.5%） （P〈0.05, OR=0.297）. Moreover, the frequency of the HLA-DQB1＊05 allele in the Uygur group with severe caries （37.5%） was significantly higher than in the control group （17.5%） （P〈0.05, OR=2.829）. Conclusion Caries susceptibility among the Uygur and Han children in Xinjiang is related to the HLA-DQB1 allele. The HLA-DQB1＊02 allele may protect against caries among the Han children, whereas the HLA-DQB1＊05 allele may be responsible for the susceptibility of the Uygur children to caries.