镜检法和胶体金法在疟原虫检测中的应用评估

Practical evaluation of Microscopic examination and Colloidal gold method in detection of plasmodium

目的分析和评价镜检法和胶体金法在疟原虫检测中的应用价值。方法对2011-2015年三门峡市共62份疑似疟疾患者的血液样本分别采用镜检法和胶体金法同时进行疟原虫检测。结果镜检法和胶体金法的阳性率分别为40.32%和37.10%,经统计学分析,两者差异无统计学意义(χ2=0.25,P>0.05);两种方法的总符合率为93.54%,灵敏度和特异度分别为96.15%、100.00%;88.46%、100.00%。结论镜检法和胶体金法对疟原虫的检测结果有良好的一致性,均具有较好的临床适用性。

Objective To analyze and evaluate the clinical application value of Microscopic examination and Colloidal gold method in testing plasmodium. Methods From 2011 to 2015 in Sanmenxia 62 blood samples of suspected malaria patients were detected by used Microscopic examination and Colloidal gold method in the same time. Results The positive rate of Microscopic examination and Colloidal gold method were 40.32 % and 37.10 %, Statistical analysis , there were no significant differences between two methods （X^2=0.25,P〉0.05）,The total coincidence rate of two methods was 93.54 %. The sensibility and specificity of two methods were 96.15 % , 100.00 %; 88.46 %, 100.00 %. Conclusion Both Microscopic examination and Colloidal gold method showed good coincidence in detecting plasmodium, and two methods can be used in clinical diagnosis well.