新疆野扁桃TP-M13-SSR引物的多态性分析

Polymorphism Analysis of TP-M13-SSR Primer for Prunus tenella Batsch.

以来自5个居群的96份新疆野扁桃叶片为试材,采用TP-M13-SSR技术和毛细管荧光电泳检测的方法,分析新疆野扁桃TP-M13-SSR引物的多态性,以期为该珍稀植物的保育工作提供参考依据。结果表明:新疆野扁桃TP-M13-SSR引物库中包含23 627条序列,共有5类重复类型,以二核苷酸类型数量最多,五、六核苷酸数量极少。将12对核心引物与96份样品一同扩增后,等位基因数(No.of allele)均为2个,有效等位基因数(Ne)、观测杂合度(Ho)、期望杂合度(He)、固定指数(F)、香农指数(I)、特异等位基因(Na)、多态性信息含量指数(PIC)、标记指数(MI)、Nei′s多样性指数、遗传相似性系数(Gst)、基因流(Nm)的范围分别为:3.531 0~9.000 0、0~0.666 7、0.72~0.89、0.25~1.00、1.48~2.44、8~18、0.669 0~0.879 0、5.35~15.82、0.219 5~0.430 8、0.002 0~0.042 6、34.756 0~252.196 4。这12对引物中,引物PT-9的多态性最好,引物PT-1多态性最差。

In this study,96 sample of leaves which were selected from 5 populations of Prunus tenella Batsch.were used as materials.The TP-M13-SSR technology and capillary fluorescent electrophoresis was used to analysis the polymorphism of it.By this way,it could provide the theoretical basis for the protection and conservation of this rare plant.The results showed that five types of repeat types and23 627 sequences in the primers library.Dinucleotide was the largest,and pentanucleotide and hexanucleotide were the least.Using the 12 pairs core primers amplified with 96 samples,all of the No.of allele was 2.It was calculated that the number of effective alleles（Ne）,observed heterozygosity（Ho）,expected heterozygosity（He）,fixation index（F）,Shannon′s information index（I）,the number of different alleles（Na）,polymorphism information index（PIC）,labeling index（MI）,Nei′s deversity index,genetic similarity coefficient（Gst）and gene flow（Nm）ranged from 3.531 0-9.000 0,0-0.666 7,0.72-0.89,0.25-1.00,1.48-2.44,8-18,0.669 0-0.879 0,5.35-15.82,0.219 5-0.430 8,0.002 0-0.042 6,34.756 0-252.196 4,respectively.The polymorphism of primer PT-9 was best,and primer PT-1 was the worst.