摘要
以2年生曼地亚红豆杉幼苗为试材,探究了在模拟酸雨胁迫条件下,不同浓度(0.10、0.25、0.50、0.75mmol·L^(-1))的外源NO(nitric oxide)供体SNP(亚硝基铁氰化钠)溶液对幼苗叶片抗氧化系统的影响。结果表明:施用pH 3.0模拟酸雨会使曼地亚红豆杉幼苗叶片中丙二醛(MDA)含量和超氧阴离子(O·2)生成速率显著升高,还原型谷胱甘肽(GSH)、抗坏血酸(ASA)和类胡萝卜素含量,过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、多酚氧化酶(PPO)、抗过氧化物酶(APX)和谷胱甘肽还原酶(GR)活性显著降低。施用0.25mmol·L^(-1)SNP溶液后,MDA含量和O·2生成速率显著降低,GSH、ASA和类胡萝卜素含量显著提高,POD、SOD、CAT、PPO、APX和GR活性显著提高。综合比较得出,0.25mmol·L^(-1)的SNP溶液能有效提高曼地亚红豆杉幼苗抗酸雨胁迫能力。
Two-year-old seedlings of Taxus media was used as material,the effect of different concentrations（0.10,0.25,0.50,0.75 mmol·L^-1）of exogenous NO donor SNP on the antioxidant system of Taxus media seedlings under simulated acid rain stress were studied.The results showed that under the simulated acid rain（pH 3.0）stress the leaf malonadehyde（MDA）contents and superoxide radical（O2·^-）production rate in leaves of Taxus mediaincreased significantly;the contents of glutathione（GSH）,ascorbic acid（ASA）and carotenoids decreased obviously;the activities of peroxidase（POD）,superoxide radical（SOD）,catalase（CAT）,polyphenol oxidase（PPO）,ascorbate peroxidase（APX）and glutathione（GR）decreased markly.The exogenous NO（0.25 mmol·L^-1 SNP）could significantly decrease the leaf malonaldehyde（MDA）content and superoxide radical（O2·^-）production rate,but increase glutathione（GSH）,ascorbic acid（ASA）and carotenoids contents.It could also improve the activities of peroxidase（POD）,superoxide radical（SOD）,catalase（CAT）,polyphenol oxidase（PPO）,ascorbate peroxidase（APX）and glutathione（GR）significantly.In conclusion,the exogenous NO could effectively improve the resistance of Taxus media seedling under the simulated acid rain stress,the most pronounced treatment concentration of SNP was 0.25 mmol·L^-1.
出处
《北方园艺》
CAS
北大核心
2018年第2期83-90,共8页
Northern Horticulture
基金
国家自然科学基金资助项目(31270740)
