微藻生长期辐射特性测量方法

Experimental method of the radiative properties of microalgae at different growth period

为提高微藻光生物反应器的效率以降低成本及进行规模化培养,提出一种在线测量微藻细胞全生长期辐射特性的方法.采用定制的平板式光生物反应器,给出一种微藻生长期辐射特性在线测量方案,分析传统测量方法应用于生长期微藻辐射特性测量的局限性,基于微藻细胞辐射特性的特征提出改进方案,并以普通小球藻为例进行生长期辐射特性的实验研究.结果表明,提出的辐射特性在线测量方案可以有效地应用于微藻时间相关辐射特性的测量,改进的光谱吸收系数及衰减系数测量方法可以更准确地应用于生长过程中细胞浓度变化时辐射特性的测量,普通小球藻的可见光波段光谱吸收截面和光谱衰减截面随时间变化显著.本研究为微藻生长期辐射特性的测量提供了一种方便可行的实验方案.

To improve the efficiency of microalgae photobioreactor to reduce cultivation costs and carry out largescale cultivation,an online measurement method was proposed to measure the radiative properties of microalgae at whole growth phase. Using a custom flate-plate photobioreactor,an online measurement scheme for the radiative properties of microalgae was presented and the limitation of traditional measurement method was studied. An improved scheme was proposed based on the characteristics of radiative properties of microalgae. Chlorella vulgaris was taken as an example and its radiative properties at growth phase was experimentally studied. The results show that the proposed online measurement scheme can be effectively applied to measure the time-dependent radiative properties of microalgae. The improved scheme of spectral absorption and extinction coefficient measurement are more accurately applied to measure the radiative properties of microalgae at different growth phase with varied cell concentration. The spectral absorption and extinction corss-sections of Chlorella vulgaris vary significantly with growth time in the visible spectral range. This work provides a convenient and feasible experimental scheme for the measurement of radiative properties of microalgae at different growth period.