3-硝基丙酸诱导小鼠卵巢氧化应激模型的建立

Establishment of Ovary Oxidative Stress Model Induced by 3-Nitropropionic Acid in Mice

通过腹腔注射3-硝基丙酸(3-NPA),建立并评价小鼠卵巢氧化应激模型。雌性小鼠分为对照组和模型组,模型组腹腔注射3-NPA(20mg/kg),每日1次,对照组注射等量生理盐水。注射14d后观察小鼠增重、卵巢指数、卵泡发育、排卵数、生育能力和生发泡期(GV期)卵细胞线粒体分布情况,检测卵巢活性氧(ROS)和丙二醛(MDA)浓度,超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性,血清雌二醇(E_2)和孕酮(P_4)浓度。结果表明,通过腹腔注射3-NPA,可显著提高小鼠卵巢ROS和MDA浓度,显著增加抗氧化酶SOD、GSH-Px、CAT活性,表明卵巢氧化应激水平升高,导致闭锁卵泡比率显著增加,排卵数和生育能力显著降低,血清E_2和P_4水平显著降低,GV期卵细胞线粒体分布异常率显著增加。说明3-NPA可成功诱导建立小鼠卵巢氧化应激模型。

The study was carried out to establish and evaluate an ovarian oxidative stress model by intraper-itoneal injection of 3-nitropropionic acid （3-NPA） in mice. Fenlale mice were divided into control group andmodel group. The model group was injected intraperitoneally zith 3-NPA （20 mg/kg） once a day. The con-trol group was injected with the same amount of saline. The body weight, ovary index, follicular develop-ment,number of ovulation, fertility and the distribution of rlitochondria in germinal vesicle oocytes wereobserved after 14 days of injection. The Ovarian levels of RCIS and MDA,the activities of antioxidant en-zymes SOD,GSH-Px,CAT,the serum levels of estradiol （E2） and pregnendione （P4） were measured. Theresults showed that intraperitoneal iniection of 3-NPA can silgnificantly increase the ovarian ROS and MDAconcentrations in mice,as well as the activities of antioxidant enzymes SOD,GSH-Px and CAT, these datasuggest that ovarian oxidative stress levels increased,which result in a significant increase in atresia follicleratio and decrease of ovulation and fertility. And the oxidati＇ve stress of ovary also result in a significant de-crease of serum E2 and P4 levels and increase of abnormal rate of mitochondrial distribution in GV stage oo-cytes. 3-NPA could induce the establishment of mouse ovarian oxidative stress model.