苦参素与顺铂合用对人肝癌裸鼠皮下移植瘤组织VEGF、CD31表达的影响和意义

Oxymatrine combined with cisplatin enhances expression of vascular endothelial growth factor and CD31 in subcutaneous xenografts of human hepatocellular carcinoma in nude mice

目的分析苦参素与顺铂合用对人肝癌裸鼠皮下移植瘤组织血管内皮生长因子(vascular endothelial growth factor,VEGF)、血小板-内皮细胞黏附分子(CD31)表达的影响及临床意义。方法建立40只人肝癌裸鼠模型,随机分为4组(对照组、苦参素组、顺铂组、联合组),采用腹腔注射方式给药,给予对照组裸鼠注射生理盐水,给予苦参素组裸鼠注射苦参素,给予顺铂组大鼠注射顺铂,给予联合组裸鼠注射苦参素和顺铂,给药结束后第15天将4组裸鼠处死,取皮下移植瘤组织,对比4组抑瘤率,采用免疫组织化学染色法检测4组裸鼠皮下移植瘤组织中VEGF、CD31的表达情况。结果联合组裸鼠的肿瘤体积小于其他3组裸鼠,差异具有统计学意义(P<0.05)。VEGF阳性率检出率为20.00%,低于苦参素组的80.00%、顺铂组的70.00%、对照组的100.00%,差异具有统计学意义(P<0.05)。联合组裸鼠的CD31阳性率检出率为30.00%,低于苦参素组的90.00%、顺铂组的70.00%、对照组的100.00%,差异具有统计学意义(P<0.05)。结论苦参素与顺铂合用能够抑制人肝癌皮下移植瘤组织中VEGF、CD31的表达,延缓肿瘤生长。

ObjectiveTo study the effect of oxymatrine combined with cisplatin on the expression of vascular endothelial growth factor （VEGF） and plateletendothelial cell adhesion molecule CD31 in human hepatocellular carcinoma xenografts in nude mice. MethodsNude mice bearing human hepatocellular carcinoma xenografts were randomly divided into 4 groups for intraperitoneal injections with normal saline （control）, cisplatin, oxymatrine, or both oxymatrine and cisplatin. Fifteen days after the injections, the mice were sacrificed for examination of the expression of VEGF and CD31 in the subcutaneous xenografts with immunohistochemical staining. ResultsIn the tumorbearing mice treated with both oxymatrine and cisplatin, the tumor volume was significantly smaller than that in the other 3 groups （P〈0.05）, and the positive rate for VEGF in the subcutaneous xenograft was 20%, significantly lower than the rate of 80% in oxymatrine group, 70% in the cisplatin group and 100% in the control group （P〈0.05）. The positive rate of CD31 in the xenograft was 30% in the combined treatment group, also significantly lower than the rates in matrine group （90%）, cisplatin group （70%） and control group （100%） （P〈0.05）. ConclusionOxymatrine combined with cisplatin can inhibit the expression of VEGF and CD31 in subcutaneous xenografts of human hepatocellular carcinoma and delay tumor growth in nude mice.