期刊文献+

二至丸对D-半乳糖诱导大鼠肾细胞衰老的保护作用 被引量:14

The Protective Function of Erzhiwan on D-galactose-Induced Senescence in Normal Rat Kidney Cells
下载PDF
导出
摘要 目的探讨二至丸对D-半乳糖(D-galactose,D-gal)诱导的大鼠肾(NRK)细胞衰老的保护作用。方法使用不同浓度D-gal(1、5、10、20、40g/L)作用不同时间(24、48、72h)诱导NRK细胞衰老,β-半乳糖苷酶(β-galactosidase)染色法鉴定细胞衰老;荧光素二-β-D-吡喃半乳糖苷(Fluorescein di-β-D-galactopyranoside,FDG)法检测衰老相关的β-半乳糖苷酶活性;噻唑蓝(MTT)法检测细胞增殖活力;以GSH-PX、SOD活力与MDA含量检测细胞氧化应激水平;构建体外NRK细胞衰老模型。根据FDG的结果来评估不同浓度二至丸(0.01、0.1、1g/L)处理对衰老NRK细胞的影响,检测GSH-PX、SOD活力与MDA含量的变化来探讨二至丸对衰老NRK细胞的保护作用。结果与对照组相比,模型组(20g/L D-gal处理48h)β-半乳糖苷酶染色阳性率明显增加,β-半乳糖苷酶活性明显增加;GSH-PX、SOD活力下降,MDA含量增加(P<0.05~0.01),细胞活性无明显变化。与模型组相比,给药组β-半乳糖苷酶含量明显降低,阳性染色率显著下降,GSH-PX、SOD活力升高,MDA含量明显降低(P<0.05~0.01)。结论建立D-gal诱导的NRK细胞衰老模型;二至丸能够减轻D-gal诱导的NRK细胞衰老的程度,其保护效应的产生可能与二至丸抗氧化应激效应相关。 OBJECTIVE To investigate the protective function of Erzhiwan on Normal Rat Kidney (NRK) cells senescence induced by D-galactose (D-gal). METHODS Different concentrations of D-gal (1, 5, 10, 20, 40 g/L) were added into the cul- ture medium for different processing time (24, 48, 72 h) to induce NRK cells senescence. Cellular senescence was described by senescence-associated β-galactosidase staining and cell viability was evaluated by MTT assay. Fluorescein di-β-D-galactopyrano- side (FDG) method was used to detect the quantity of β-galactosidase. The levels of SOD, MDA and GSH-PX in the superna- tant were measured to show the changes of oxidative stress. The establishment of the NRK cell aging model was determined according to the outcome of these detections. After that, different concentrations of Erzhiwan (0.01, 0.1, 1 g/L) were added into the culture medium, and the effect of Erzhiwan on the aged NRK cell was observed according to the outcome of FDG de- tection. The activity of SOD and GSH-PX, the content of MDA were measured. RESULTS Compared with control group, the number of β-galactosidase-positive cells in model group (20 g/L D-gal exposure for 48 h) was apparently increased. In mod- el group, the activity of β-galactosidase detected by FDG was markedly increased, and the activity of SOD, GSH-PX was down -regulated while the content of MDA in the cell culture supernatant was increased. No significant change of the ceil viability be- tween the 2 groups was observed. Compared with model group, the activity of β-galactosidase in Erzhiwan treatment group was decreased. Erzhiwan also up-regulated the activity of SOD, GSH-PX and decreased the content of MDA in the cells. CON- CLUSION Up-regulation of oxidative stress level exists in D-gal-induced aged NRK cells. Erzhiwan attenuates D-gal-induced senescence in NRK cells.
出处 《南京中医药大学学报》 CAS CSCD 北大核心 2018年第1期81-86,共6页 Journal of Nanjing University of Traditional Chinese Medicine
基金 国家自然科学基金(81573554) 江苏高校“青蓝工程”项目 江苏省“六大人才高峰”高层次人才项目(YY026)
关键词 D-半乳糖 二至丸 大鼠肾细胞 细胞衰老 氧化应激 D-galactose Erzhiwan Normal Rat Kidney cell cell aging oxidative stress
  • 相关文献

参考文献5

二级参考文献88

共引文献146

同被引文献211

引证文献14

二级引证文献132

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部