MACC1在卵巢癌组织中表达及对卵巢癌细胞生物学特性的影响

The expression of MACC1 in ovarian cancer and its effect on the biological characteristics of ovarian cancer cells

目的探讨结肠癌转移相关基因(MACC)1在卵巢癌组织中的表达及对卵巢癌细胞生物学特性的影响。方法收集卵巢癌组织及对应的癌旁组织,Western印迹检测组织中MACC1表达水平。以卵巢癌细胞A2780为研究对象,细胞转染MACC1小干扰RNA(MACC1 siRNA)、siRNA对照(siRNA control),细胞分为未转染组(只加入转染试剂)、siRNA control组(转染siRNA control)、MACC1 siRNA组(转染MACC1 siRNA)。培养24 h后,Western印迹检测细胞中MACC1、基质金属蛋白酶(MMP)-2、MMP-9表达水平,噻唑蓝(MTT)检测细胞增殖,细胞划痕实验检测细胞迁移能力,Transwell小室检测细胞侵袭能力。结果卵巢癌组织中MACC1表达水平高于癌旁组织。siRNA control组细胞中MACC1、MMP-2、MMP-9表达水平及细胞存活率、迁移率、侵袭细胞数目与未转染组相比均没有差异,MACC1 siRNA组细胞中MACC1、MMP-2、MMP-9表达水平及细胞存活率、迁移率、侵袭细胞数目均明显低于未转染组。结论 MACC1在卵巢癌组织中过度表达。干扰MACC1能够抑制卵巢癌细胞增殖、迁移、侵袭能力,作用机制可能与抑制MMP-2、MMP-9表达有关。

Objective To investigate the expression of MACC1 in ovarian cancer and its effect on the biological characteristics of ovarian cancer cells. Methods Ovarian cancer tissues and corresponding adjacent tissues were collected, MACC1 expression in tissues was detected by Western blot. Ovarian cancer cell line A2780 was used as the research object, transfection of MACC1 small interfering RNA （MACC1 siRNA）, siRNA control （siRNA control）, cells were divided into non transfection （with transfection reagent）, siRNA control （transfected with siRNA control ）, MACC! siRNA groups（ transfected with MACC1 siRNA）. After culturing 24 h, Western blot was used to detect the expression levels of MACC1 ,MMP-2 and MMP-9 in the cells,cell proliferation was detected by MTY,cell scratch assay was used to detect the ability of cell migration,the invasion ability of Transwell cell assay. Results The expression of MACC1 in ovarian cancer tissues was higher than that in adjacent tissues. The expression levels of MACC1, MMP-2, MMP-9 and cell survival rate, migration rate and the num- ber of invasive cells in siRNA control group were not different from those in non transfection group. The expression levels of MACC1, MMP-2, MMP-9 and cell survival rate, migration rate and invasive cell number in MACC1 siRNA group cells were significantly lower than those in non transfection group. Conclusions Interference of MACC1 could inhibit the proliferation, migration and invasion of ovarian cancer cells, and the mechanism might be related to the inhibition of MMP-2 and MMP-9 expressions.