高温环境下湖羊直肠温度和血液生化指标的监测

Monitoring of Rectal Temperature and Blood Indexes of Hu Sheep under High Ambient Temperature

本研究以南方特色绵羊品种湖羊为研究对象,在夏季高温环境下,连续监测10d湖羊直肠温度变化,测定血常规指标,并比较不同年龄、不同性别的湖羊在高温环境下血常规指标的差异;以湖羊高温环境下直肠温度的变化为基础,结合血常规指标,在湖羊群体内鉴别高温应激敏感和高温应激抗性湖羊个体。结果显示,湖羊最高直肠温度出现在环境温度最高的时段(11∶00~15∶00),最低温度一般出现在03∶00左右,且以24h为周期循环;平均最高直肠温度和最低直肠温度差在湖羊个体间差异显著(P<0.05);高温暴露后湖羊群体淋巴细胞(LYM)、红细胞(RBC)、血红蛋白(HGB)数量及平均血红蛋白浓度(MCHC)均超出了绵羊正常参考值,且在不同性别和年龄上有一定的差异:母羊嗜碱性细胞、红细胞、血红蛋白、红细胞压积均有高于公羊的趋势,但差异不显著(P>0.05);1周岁湖羊淋巴细胞(P>0.05)、嗜碱性细胞计数(P<0.01)及百分比(P<0.05)均高于半岁湖羊;依据温度变化结合血常规指标变化在湖羊群体中鉴别了高温应激敏感和抗性的湖羊个体,在测试的母羊群体中高温应激敏感湖羊占35.3%,高温应激抗性湖羊占26.5%。肌酸激酶(CK)含量在高温应激敏感和抗性湖羊个体间差异显著(P<0.05)。综合以上结果,高温环境下湖羊不同个体间直肠温度变化差异显著;从血常规指标来看,高温应激对母羊和1岁湖羊的影响比较大。试验结果为深入研究夏季高温应激对湖羊的影响及抗高温应激差异的分子机制提供了一定的依据。

The continuously changes of rectal temperature and blood biochemical indexes were monitored in 10 din Hu sheep（a southern special sheep breed）exposed to high ambient temperature.Changes of rectal temperature and blood biochemical indexes were analyzed statistically,based on these data,sensitive individuals and resistant individuals were separated in Hu sheep under high temperature.The results showed that the highest rectal temperature of Hu sheep appeared at the highest ambient temperature（11∶00-15∶00）,the lowest rectal temperature generally appeared at about 03∶00 and the rectal temperature was circulated in 24 hcycles.The mean difference between the highest temperature and the lowest temperature was significant（P 0.05）.After heat stress,the lymphocyte（LYM）,red blood cell（RBC）,hemoglobin（HGB）,and mean hemoglobin（MCHC）were above the normal values for sheep,which were influenced by both sex and age.Basophilic cell（BAS）,RBC,HGB,hematocrit（HCT）of ewes were higher than those of rams,but the differences were not significant（P〈0.05）.The LYM（P〈0.05）,BAS count（P〈0.01）and percentage（P〈0.05）of one year old Hu sheep were higher than those of half a year old.Mainly based on changes of rectal temperature and blood biochemical indexes,high temperature stress sensitive sheep and resistance individuals were identified in Hu sheep,and high temperature stress sensitive individuals counted 35.3%,high temperature stress resistant individuals counted 26.5%.Creatine kinase（CK）content was significantly different between sensitive individuals and resistant individuals（P〈0.05）.In conclusion,the rectal temperature of Hu sheep was significant difference under high environment temperature,and ewes and one year old sheep were sensitive to heat,these results would provide a basis for better to study the principles and molecular mechanism of heat stress in sheep.