抗松针褐斑病湿地松组培再生植株抗病性测定

Determination of resistance to brown-spot needle blight on culture seeding of slash pine

采用松针褐斑病菌毒素处理湿地松瓶内组培苗和温室组培苗针叶、采用苗圃组培苗人工接种松针褐斑病菌孢子悬浮液测定抗病性。结果表明:湿地松不同家系间抗病性差异显著、同一家系不同无性系间抗病性差异也显著。湿地松瓶内组培苗各无性系抗病性强弱依次为8#40,8#2,8#11,32#3,普通组培苗;温室组培苗各无性系抗性强弱依次为32#7,32#3,普通组培苗。苗圃组培苗与实生苗间、组培苗不同代数间抗病性差异显著。单元回归分析表明,瓶内测定结果与温室针叶测定结果的相关系数r^2=0.999 7、与苗圃接种孢子悬浮液测定结果的相关系数r^2=0.958 8。因此,为了缩短选育时间,减少成本,一定程度上可以用瓶内抗病性测定替代室外抗病性测定。

Resistance of plant regeneration of Pinus elliottii treated by the toxin of Lecanosticta acicola in bottle and greenhouse and that after inoculating spore suspension of L. acicola in nursery were determined. The results showed that the disease resistance had significant differences among different families and among different clones of the same family. The strength order of resistance of clones in bottle were 8#40,8#2,8#11,32#3,normal tissue culture,while the order in greenhouse were 32#7,32#3,normal tissue culture. Differences of resistance between tissue culture and seedling and among different generations of tissue culture were significant. Simple regression analysis showed that the results in bottle were relevant to the results in greenhouse and nursery,and r^2 were 0. 999 7 and 0. 958 8 respectively.So in order to shorten the breeding time and reduce the costs,determination of disease resistance of the clones in bottle could be an alternative to outdoor resistance test to a certain extent.