竹叶中叶绿素的提取工艺及其功能性应用研究

Research on the extraction and functional application of chlorophyll from bamboo leaves

以毛竹叶为原料,使用丙酮作为提取剂,通过振荡水浴预处理,辅助超声波作用提取毛竹叶叶绿素,并且对叶绿素提取效率进行测试。对主要参数(如振荡时间、超声时间、超声功率等)设计了单因素试验。得出较适宜的提取工艺为:振荡水浴处理时间10min,超声波清洗器处理时间20min,超声功率140W,在此条件下,叶绿素提取效率较高,提取含量达到2.39mg/g。同时,采用不同方法制备聚乙烯醇(PVA)/叶绿素膜,通过荧光显微镜、扫描电子显微镜(SEM)对制得PVA/叶绿素膜进行观测。结果表明,PVA/叶绿素膜保持叶绿素的荧光特性;在纺丝工艺中,叶绿素吸附在纤维表面形成PVA/叶绿素膜。

The bamboo leaves were subjected to acetone solvent for chlorophyll extraction.The reaction was carried out in the shaking water bath prior to the ultrasonic cleaner treating.The chlorophyll extraction efficiency was investigated.Moreover,in order to acquire favorable main values,the single-factor experiments was designed for main parameters,viz.treating time of shaking water bath as well as temperature and power of ultrasonic cleaner.The proper parameters for launching the process were：shaking water bath treating time of 10 min,ultrasonic cleaner extraction time of 20 min and power of 140 W.Under this condition,higher extraction efficiency could be achieved,which was 2.39 mg/g.Additionally,PVA/chlorophyll films prepared through different methods were observed by fluorescence microscopy and SEM.Results indicated that PVA/chlorophyll film retained the fluorescence characteristics of chlorophyll.In the spinning process,chlorophyll was absorbed on the surface of fibers forming PVA/chlorophyll film.