小桐子MYB基因家族的鉴定及JcMYB308基因的克隆与低温表达分析

Genome-wide Identification of MYB Gene Family and Cloning & Expression Analysis of JcMYB308 Gene from Jatropha curcas

MYB转录因子家族参与植物生长发育及对环境胁迫的应答等过程。该实验基于小桐子基因组数据库,对MYB基因家族进行鉴定,克隆了小桐子Jc MYB308基因,并对其功能结构域、系统进化、基因结构及低温表达特性进行了分析。结果表明,小桐子全基因组共鉴定到213个MYB基因家族成员,聚类为6个亚家族。克隆的Jc MYB308基因片段长度为713 bp,基因结构具有较高的保守性,均含有两个外显子,进化树显示其与同属大戟科的蓖麻亲缘关系最近,序列一致性为62.7%。q RT-PCR表达分析表明,小桐子Jc MYB308基因的表达存在组织表达特异性,在根中表达量较高,而在叶片中表达量相对较低,在根与茎中低温胁迫24 h时达到最大表达量。

MYB transcription fatctor family inwdves in the processes of the plant growth and development and the response to environmental stresses. Based on the database of Jatropha curcas genome, MYB308 gene family was identified, then JcMYB308 of J. curccts was cloned, and the functional domains, phylogenetie relationship, gene structure, and ehilling expression eharacteristies were analyzed. The results demonstrated that the whole genome was identified 1o have 213 memhers of gene family, and they were elustered as 6 subfamilies. The cloned JcMYB308 gene fragmen! length was 713 hp, and the gene strueture was highly eonserved, with two exons. In addition, the phylogenetie tree showed that its amino acid sequenee shared 62.7% idenlily with Ricinus cornmunis lhat behmged to Ihe same family of Euphorbiaceae. qRT-PCR expression analysis revealed that JcMYB308 expressed in different organs, abundantly in root, hut scarcely in leaves, and at the highest level in stem and root at 24 h after cold-induction.