羊耳蒜化学成分的研究

Chemical Constituents from Liparis japonica

目的研究羊耳蒜(Liparis japonica)全草的化学成分。方法采用硅胶、ODS、Sephadex LH-20等柱色谱进行分离纯化,通过波谱学方法及与文献对照鉴定化合物结构。结果分离得到18个化合物,分别鉴定为acortatarin A(1),3β-hydroxy-5α,8α-epidioxyergosta-6,22-diene(2),13-epicupressic acid(3),3β-hydroxyolean-12-en-28-oic acid(4),白桦脂酸(5),gigantol(6),(2S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D-galactopyranoside(7),α-linolenic acid(8),hydroxydihydrobovolide(5-hydroxyl-3,4-dimethy-5-penty-12(5H)-furanone)(9),反式对羟基肉桂酸(10),(+)-丁香脂素(11),β-谷甾醇(12),2,5-二羟基-4-甲氧基-9,10-二氢菲(13),2,7-二羟基-3,4-二甲氧基菲(14),moscatin(15),山药素Ⅲ(16),亚油酸(17)和对羟基苯甲醛(18)。结论本实验首次从羊耳蒜属植物中分离得到结构独特的含吗啉片段的螺环生物碱,化合物2~11为首次从该属植物中分离得到。

OBJECTIVE To study the chemical constituents of the whole herb of Liparis japonica. METHODS The compounds were isolated by column chromatography on silica gel, ODS and Sephadex LH -20 columns. Their structures were identified by analy- sis of physical and spectroscopic data and confirmed by comparison of their spectral data with those in the literature. RESULTS Eighteen compounds were isolated and identified respectively as acortatarin A （1）, 3αhydroxy-5α, 8α-epidioxyergosta-6,22-diene （ 2 ）, 13-epicupressic acid （ 3 ）, 3β-hydroxyolean-12-en-28-oic acid （4）, betulinic acid （ 5 ）, gigantol （6）, （2S） -3-O-octadeca-9Z, 12Z, 15Z-trienoylglyceryl-O-β-D-galactopyranoside （ 7 ）, linolenic acid （ 8 ）, hydroxydihydrobovolide （ 5-hydroxyl-3,4-dimethy-5-penty- 12（5H）-furanone） （9）, trans-p-hydroxycinnamic acid （10）, （ ＋ ）-syringaresinol （11）, β-sitosterol （12）, hircinol （13）, nudol （14）, moscatin （15）, batatasin III（16）, linoleic acid （17）, and p-hydroxybenzaldehyde （18）. CONCLUSION We obtained structurally unique spiroalkaloid with morphine structure from the genus Liparis. Compounds 2 - 11 are isolated for the first time from this genus for the first time.