地龙降压胶囊对自发性高血压大鼠肾胺酶表达的影响

Effect of Dilong antihypertensive capsule on expression of renalase in spontaneously hypertensive rats

目的:观察地龙降压胶囊对自发性高血压大鼠(SHR)肾胺酶表达的影响,以探讨地龙降压胶囊治疗原发性高血压的作用机制。方法:取24只8周龄雄性SHR,根据收缩压随机分为模型组(等容量蒸馏水灌胃)及地龙降压胶囊高、低剂量组(分别以地龙降压胶囊240mg/kg、120mg/kg灌胃),每组8只。另取8只WKY雄性大鼠作为正常对照组(等容量蒸馏水灌胃)。分别于连续给药前、给药后第2周、4周、6周、8周测定尾动脉收缩压。末次给药后,采用ELISA法检测大鼠血浆儿茶酚胺和血清肾胺酶含量。采用免疫组织化学法检测大鼠肾脏肾胺酶的表达。结果:地龙降压胶囊有效降低了SHR收缩压、血浆儿茶酚胺水平(P<0.05);升高了SHR肾胺酶水平(P<0.05)。结论:地龙降压胶囊能有效降低血压,其作用机制可能是通过调节肾胺酶表达水平,从而影响血浆儿茶酚胺水平实现的。

Objective：To observe the effect of Dilong Antihypertensive Capsule on the expression of renalase in spontaneously hypertensive rats（SHR）,and explore the underling mechanisms.Method：The 24 male SHRs of 8 weeks old were randomly divided into model group（gavage of distilled water of the same volume）,high dose group,low dose group（240 mg/kg,120 mg/kg）,8 rats in each group.Another 8 WKY male rats were taken as normal control group（gavage of distilled water of the same volume）.The carotid systolic blood pressure was measured at 2 weeks,4 weeks,6 weeks and 8 weeks after the continuous administration.After the last administration,the levels of plasma catecholamines and serum renalase were measured by ELISA.Expression of renalase in Kidney was detected by Tissue Chemistry.Result：Dilong Antihypertensive Capsule effectively reduced the SHR systolic blood pressure,plasma catecholamine levels（P〈0.05）;increased the level of SHR renalase（P〈0.05）.Conclusion：Dilong Antihypertensive Capsules can effectively reduce blood pressure and its mechanisms may be through regulating the expression level renalase,and then affecting plasma catecholamine levels.