硅胶固相萃取-高效液相色谱法测定植物油中的特丁基对苯二酚含量

Determination of tert-butylhydroquinone content in vegetable oil by silica gel solid phase extraction-high performance liquid chromatography

建立了硅胶固相萃取-高效液相色谱测定植物油中特丁基对苯二酚(Tert-Butylhydroquinone,TBHQ)含量的方法。该实验对硅胶的用量、油样量、洗脱剂的极性、流速和层析柱规格进行了优化组合。分别用3.1μg、5.4μg和13.6μg作加标回收率和精密度试验,其回收率在95.6%~103.7%,RSD%为3.5%~5.1%,且同一样品的TBHQ含量测定结果与NY/T1602—2008测定的基本一致,说明该方法具有良好的准确性和重复性,可用于植物油中TBHQ含量的测定。

A silica gel solid phase of the neutral alumina extraction-high performance liquid chromatographic method was established for the analysis of Tert-Butylhydroquinone （Tert-Butylhydroquinone,TBHQ） in the Vegetable oil. The amount of silica gel, oil, the polarity of the eluent, the velocity and the specifications of chromatography col- umn were optimized in this experiment. The recovery rate and precision test experiments showed that： the recovery rate for 3.1 p,g,5.4 μg and 13.6 μg were 95. 6% -103.7%, RSD % were 3.5% -5.1%. The results of TBHQ content in the same sample were basically the same as that of NY/T1602--2008. This indicates that the method has good a accuracy and repeatability. It can be used for the determination of TBHQ content in vegetable oil.