臭氧水疗治疗变应性接触性皮炎样小鼠的疗效观察

Clinical Observation of Ozone Hydrotherapy on Mouse Model with Allergic Contact Dermatitis

目的观察臭氧水疗治疗变应性接触性皮炎样小鼠的疗效。方法 DNCB诱导的变应性接触性皮炎样小鼠模型40只,随机分成A组(臭氧水疗组),B组(药物治疗组),C组(纯净水治疗组),D组(自然恢复组)。A、B、C三组皮损处分别予以臭氧水疗、外用药物、纯净水治疗,连续治疗2周,D组不做任何处理。2周后处死小鼠,HE染色观察各组小鼠的皮肤组织病理改变;透射电镜观察各组小鼠皮肤棘层的变化;实时定量PCR(RT-PCR)检测皮损中γ-干扰素(IFN-γ)、白介素-10(IL-10)、白介素-17(IL-17)表达水平。结果 A组及B组较C组及D组小鼠的搔抓症状及皮炎表现好转,皮损处病理结果与电镜下超微结构明显改善。A组及B组小鼠皮损中IL-10、IL-17 m RNA水平明显低于C组和D组,IFN-γm RNA水平较C组及D组明显升高,差异有统计学意义(P<0.05)。结论臭氧水疗对治疗变应性接触性皮炎样小鼠有明显疗效,可能通过调控T细胞间的平衡及细胞的氧化还原反应发挥治疗作用。

Objective To observe the effect of ozone hydrotherapy on allergic contact dermatitis（ACD）mice.Methods Forty mice with ACD induced by dinitrochlorobenzene（DNCB）were randomly divided into four groups： group A（ozone hydrotherapy group）, group B（drug therapy group）, group C（purified water therapy group）and group D（natural recovery group）.Skin lesions of groups A, B and C were treated with ozone liquid, topical drug, purified water respectively for 2 weeks, and the group D was received no treatment.Two weeks later, the mice were killed and the skin lesions were collected and the pathology of skin tissues of each group were observed with HE dyeing; the changes of skin spines in mice were observed by electron microscopy; the expression level of Interferon-γ（IFN-γ）, Interleukin-10（IL-10）and Interleukin-17（IL-17）in the skin lesions were quantitatively detected by real-time PCR（RT-PCR）.Results Scratching symptoms, pathological results and ultrastructural of skin lesions of mice in group A and group B were better than these in groups C and D.The expression levels of IL-10 and IL-17 mRNA in skin tissues of both group A and group B were clearly lower than those of the group C and the group D; on the contrary, the level of IFN-γ mRNA of the two groups were higher than those of the latter two groups（P〈0.05）.Conclusion Ozone hydrotherapy has a significant effect on ACD mice through adjusting and controlling the imbalance of T cell and cell redox reaction.