浙江大豆核心亲本蛋白质和异黄酮含量的分析

Analysis of Protein and Isoflavone Contents in Zhejiang Soybean Core Collections

蛋白质和异黄酮是大豆的2个重要品质性状,但是这两类物质之间的关系在浙江省内却少有研究。本研究选取了种植于浙江省的196份来自不同地区(94份来自黄淮海地区,102份来自南方地区)的大豆核心亲本作为研究对象,测定其蛋白质和异黄酮组分的含量,并对蛋白质与异黄酮各组分之间进行相关性分析。结果表明,蛋白质含量和异黄酮总含量的范围分别为352~501 g/kg和254.1~6 068.9 mg/kg;丙二酰染料木苷、丙二酰黄豆苷、黄豆苷和染料木苷这4种成分的平均含量最高,分别为966.4、667.2、206.0、229.3 mg/kg。我国南方地区大豆种质的蛋白质含量显著高于黄淮海地区,但是两个地区异黄酮总含量则无显著差异。对于蛋白质与异黄酮成分的相关性分析,发现蛋白质与染料木苷和黄豆黄素苷元之间均存在负相关性。在参试材料中,响水黑豆和72-424这2个品种具有高蛋白、高异黄酮性状。大豆籽粒的蛋白质与异黄酮性状能够同时进行改良,通过筛选和育种改良大豆品质,可以获得高蛋白、高异黄酮大豆。

Protein and isoflavone are two important quality traits in soybean seeds. However,the investigations about the relationship between protein and isoflavone are still limited in Zhejiang Province for breeding soybeans with improved seed quality. In this study, a collection of 196 soybean core parents （94 from the Huang- Huai -Hal region and 102 from the South region）grown in Zhejiang were evaluated for both protein and isoflavone and the relationship between protein and isoflavone was also studied. The total of protein and isolavone contents ranged from 352 to 501 g/kg and from 254.1 to 6 068.9 mg/kg, respectively. Malonylgenistin, malonyldaidzin, daidzin, and genistin were four major components in them and their average contents were 966.4,667.2,206.0, and 229.3 mg/kg, respectively. However, the total isoflavone contents showed no difference between the Huang- Huai -Hal region and the South region in China. The total protein level had moderate negative correlations with genistin and glycitein. The two germplasms, Xiangshui black soya bean and 72 -424, exhibited both high protein and high isoflavone contents. These results provided useful information for future breeding and genetic research about seed quality improvement.