亚麻籽胶低聚糖的制备及其生物活性评价

Preparation of Flaxseed Gum Oligosaccharides and Their Bioactivity Evaluation

以亚麻籽胶为原料,采用双氧水氧化降解法制备了亚麻籽胶低聚糖(Flaxseed Gum Oligosaccharides,FGOS),并通过体外抗氧化和乳酸菌增殖实验评价了FGOS的生物活性。结果表明,双氧水氧化降解法能有效地降解亚麻籽胶,降解产物FGOS为聚合度为2~6的低聚糖,由鼠李糖、岩藻糖、阿拉伯糖、木糖、甘露糖、葡萄糖及半乳糖7种单糖组成。体外抗氧化实验结果表明FGOS具有Fe^(3+)还原能力,并与FGOS质量浓度呈正相关,脂质氧化抑制率及超氧阴离子清除率分别可达82.76%(5.0 mg/mL)及72.93%(10 mg/mL)。乳酸菌增殖实验结果表明FGOS能有效促进鼠李糖乳杆菌、发酵乳杆菌、植物乳杆菌及副干酪乳杆菌的生长,益生指数PI值分别为24.22%、18.84%、65.58%、25.51%,短链脂肪酸(SCFA)的产量分别为500.66、295.79、636.32、405.86μg/mL,可有效促进SCFA的积累。

Hydrogen peroxide（ H202 ）oxidation degradation method was applied to prepare flaxseed gum oligo- saccharides （FGOS）using flaxseed gum as the raw material. In vitro antioxidation and lactobacillus proliferation tests were conducted to evaluate the bioactivities of the obtained FGOS. The results showed that hydrogen peroxide oxidation degradation was able to efficiently degrade flaxseed gum, and the degradation product was oligosaccharides with 2 - 6 degree of polymerization which consists of rhamnose, fucose, arabinose, xylose, mannose, glucose and galactose. In vitro antioxidation experiment showed that FGOS has the reduce capacity of Fe^3＋ , and the capacity is positively correlated with FGOS mass concertration, anti - lipid - oxidation （82.76% at 5.0 mg/mL） and superoxide radical scavenging（72.93% at 10 mg/mL）. Lactic acid bacteria multiplication experiment also showed that FGOS is able to promote the growth of Lactobacillus rhamnosus, Lactobacillus fermentation, Lactobacillus plantarum and Lactobacillus paracasei. The Probiotic Index （PI） towards the four lactobacillus were 24.22 %, 18.84% ,65.58 % ,25.51% respectively and the short chain fatty acid（SCFA） were effectively accumulated with production of 500.66,295.79,636.32, 405.86μg/mL respectively.