维生素E对三黄肉鸡免疫功能、组织α-生育酚沉积及脂蛋白酯酶、脂肪酸结合蛋白基因表达的影响

Effects of Vitamin E on Immune Function,α-Tocopherol Deposition and Gene Expressions of Lipoprotein Lipase and Fatty Acid-Binding Proteins in Tissues of Sanhuang Chicken

本试验旨在研究饲粮不同维生素E水平对三黄肉鸡免疫功能、组织α-生育酚沉积及脂蛋白脂酶(LPL)、脂肪酸结合蛋白基因表达的影响。将256只80日龄广西三黄肉鸡随机分为4组,各组分别在基础饲粮中添加0(对照)、50、100、150 mg/kg的维生素E,每组4个重复,每重复16只。预试期5 d,正试期35 d。结果表明,与对照组相比:1)饲粮添加50 mg/kg维生素E显著提高血清中免疫球蛋白A含量(P<0.05);饲粮添加150 mg/kg维生素E显著提高血清中免疫球蛋白M含量(P<0.05);饲粮添加不同水平的维生素E对脾脏指数和血清中白细胞介素2、肿瘤坏死因子α、γ干扰素及免疫球蛋白G含量均无显著影响(P>0.05)。2)饲粮添加50、100及150 mg/kg维生素E均能显著提高肝脏α-生育酚含量(P<0.05);饲粮添加150 mg/kg维生素E显著提高胸肌α-生育酚含量(P<0.05);饲粮添加不同水平维生素E对腿肌α-生育酚含量无显著影响(P>0.05)。3)饲粮添加100和150 mg/kg维生素E均能显著提高肝脏LPL基因表达量(P<0.05);饲粮添加150 mg/kg维生素E能显著提高肝脏心脏型脂肪酸结合蛋白(H-FABP)基因表达量(P<0.05);饲粮添加50、100及150 mg/kg维生素E均能显著提高肝脏肝脏型脂肪酸结合蛋白(L-FABP)基因表达量(P<0.05);饲粮添加不同水平维生素E对肝脏脂肪细胞型脂肪酸结合蛋白(A-FABP)基因表达量无显著影响(P>0.05)。4)饲粮维生素E添加水平与广西三黄肉鸡肝脏LPL、H-FABP、L-FABP基因表达量呈显著正相关(P<0.05)。综合得出,饲粮添加高水平(150 mg/kg)的维生素E可以改善广西三黄肉鸡的免疫功能,提高组织中α-生育酚的沉积,调节肝脏LPL、H-FABP、L-FABP基因表达量,从而影响机体的脂质代谢。

This experiment was to investigate the effects of different dietary vitamin E levels on immune function,α-tocopherol deposition and gene expressions of lipoprotein lipase（ LPL） and fatty acid-binding proteins in tissues of Sanhuang chicken. A total of 256 Guangxi Sanhuang chickens at 80 days of age were randomly divided into 4 groups with 4 replicates per group and 16 chickens per replicate. Vitamin E at 0（ control）,50,100 and 150 mg/kg was added to a basal diet of different groups,respectively. Pre-experiment lasted for 5 d,and experiment lasted for 35 d. The results showed that compared with control group： 1） the content of immunoglobulin A（ IgA） in serum was significantly increased by dietary supplementation of 50 mg/kg vitamin E（ P0.05）; the content of immunoglobulin M（ IgM） in serum was significantly increased by dietary supplementation of 50 mg/kg vitamin E（ P0.05）; dietary supplementation of different levels of vitamin E had no significant effects on spleen index,and the contents of interleukin-2,tumor necrosis factor-α,interferon-γ and immunoglobulin G（ IgG） in serum（ P0.05）. 2） The content of α-tocopherol in liver was significantly increased by dietary supplementation of 50,100 and 150 mg/kg vitamin D（ P 0. 05）; the content of α-tocopherol in breast was significantly increased by dietary supplementation of 150 mg/kg vitamin D（ P0.05）;dietary supplementation of different levels of vitamin E had no significant effects on the content of α-tocopherol in thigh（ P0.05）. 3） The expression level of LPL gene in liver was significantly increased by dietary supplementation of 100 and 150 mg/kg vitamin D（ P0.05）; the expression level of heart type-fatty acid-binding protein（ H-FABP） gene in liver was significantly increased by dietary supplementation of 150 mg/kg vitamin D（ P0.05）; the expression level of liver type-fatty acid-binding protein（ L-FABP） gene in liver was significantly increased by dietary supplementation of 50,100 and 150 mg/kg vitamin D（ P0.05）; dietary supplementation of different levels of vitamin E had no significant effects on the expression level of adipocyte typefatty acid-binding protein（ A-FABP） gene（ P0.05）. 4） There was a significant positive correlation between dietary vitamin E level and expression levels of LPL,H-FABP and L-FABP genes in liver of Guangxi Sanhuang chicken（ P0.05）. In summary,it is suggested that the supplementation of a high level（ 150 mg/kg）of vitamin E in diet can improve immune function,increase α-tocopherol deposition in tissues,and regulate expression levels of LPL,H-FABP and L-FABP genes in liver of Guangxi Sanhuang chicken,which may has impact on the body＇s fat metabolism.