血清REG4和CA19-9在早期胃癌患者中的表达及其意义

Effect of Expression Levels of Serum Regenerating Gene Protein 4 and Carbohydrate Antigen19-9 in Patients with Early Gastric Cancer

【目的】探讨血清中再生蛋白4（REG4）和糖类抗原19—9（CA19—9）在早期胃癌患者中的表达及其意义。【方法】选择本院2015年6月至2016年6月收治并确诊为早期胃癌的患者90例为观察组，同时选择本院体检中心90例健康人员为对照组。收集两组空腹血样，采用酶联免疫吸附实验（ELISA）检测两组受试者血清REG4表达水平，并采用化学发光免疫法检测血清CA19-9表达水平。经胃镜及组织病理学检查确定肿瘤分期、组织类型、分化程度、部位等组织病理学参数。对比分析两组受试者血清REG4和CA19—9水平，并探讨观察组血清REG4、CA19—9与不同性别、年龄和肿瘤分期、分化程度等病理学参数的相关性。【结果】观察组血清REG4和CA19-9水平均显著高于对照组（P〈0．05）；Ib期患者血清REG4、CA19—9水平均显著高于Ia期者（P〈0．05）；血清REG4、cA19—9在肿瘤分化程度中分化者水平显著高于高分化者，肿瘤分化程度低分化者水平显著高于中分化者（P〈O．05）；血清REG4、CA19-9在浸润程度黏膜下层者水平显著高于浸润程度黏膜层者（P〈0．05）；肿瘤直径〉3cm者水平显著高于肿瘤直径2～3cm者（P〈0．05），肿瘤直径2～3cm者水平显著高于肿瘤直径1～2cm者（P〈0．05），肿瘤直径1～2cm者水平显著高于〈1cm者（P〈0．05）。而不同性别、年龄、组织类型及肿瘤分布部位的早期胃癌患者血清REG4、CA19—9水平无显著差异（P〉0．05）。【结论】血清REG4和CA19—9水平在早期胃癌患者中出现明显升高，与肿瘤分期、分化程度、浸润深度、肿瘤直径也有紧密的关系。据此可以综合评估患者病情。

[Objeetive]To explore the expression levels of serum regenerated gene protein 4 （REG4） and carbohydrate antigen 19-9 （CA19-9） in patients with early gastric cancer. [Methods]A total of 90 cases of patients diagnosed with early gastric cancer from June 2015 to June 2016 in our hospital were selected as the observation group and 90 healthy persons in the same time frame were selected as the control group. Blood samples from the two groups were collected. The serum REG4 expression levels were detected by enzyme linked immunosorbent assay （ELISA） and the serum CA19-9 expression levels were detected by chemiluminescence immunoassay. The histopathological parameters of tumor staging, tissue type, differentiation degree and distribution were determined by gastroscopy and histopathological examination. The serum REG4 and CA19-9 levels were then compared between the two groups. The relationships between the levels of serum REG4and CA19-9 with different gender, age and other pathological parameters in the observation group were analyzed. [ResultslThe levels of serum REG4 and CA19-9 in the observation group were significantly higher than those in the control group （ P 〈0.05）. The levels of serum REG4 and CA19-9 in stage Ib patients were significantly higher than in stage Ia patients （ P 〈0.05）. The levels of RGE4 and CA19-9 in the patients of highly differentiated were significantly higher than those in the moderately differentiated, while the patients of moderately differentiated were significantly higher than those in the low/undifferentiated （ P 〈0.05）. The levels of serum RGE4 and CA19-9 were significantly higher in the patients with tumor cells that infiltrated the submucosa than those inwith tumor cells that infiltrated the mucosa （ P 〈0.05）. The serum levels of RGE4 and CA19-9 in the patients of tumor diameter of 〉3 cm were significantly higher than in patients with tumor diameter of 2~3 cm （ P 〈0.05） the patients with tumor diameter of 2-3 cm had significantly higher than serum levels of those with tumor diameter of 1~2 cm; the patients with tumor diameter of 1~2 cm had significantly higher serum levels than those with tumor diameter of 〈1 cm（ P〈 0.05）. There were no significant differences in REG4 and CA19-9 expression with differing gender, age, tissue type and tumor distribution （ P 〉0.05）. [Conclusion]The serum REG4 and CA19-9 levels are significantly elevated in early gastric cancer patients and are elosely associated with tumor staging, differentiation degree, infiltration depth, and tumor .,size.