摘要
目的:探讨牙本质发育不良I型(dentin dyspepsia type I,DD-I)相关致病基因Ssuh2在小鼠牙胚发育各个时期中的作用。方法:采用HE确定小鼠下颌第一磨牙牙胚发育E13.5d、E14.5d、E16.5d、P2.5d、P7d的时期,采用免疫组化法观察SSUH2在各个时期的表达分布。结果:蕾状期(E13.5d),SSUH2在上皮细胞和间充质细胞均呈阳性表达;帽状期(E14.5d)和钟状早期(E16.5d),SSUH2均在基底膜、内、外釉上皮层、釉结、颈环呈强阳性表达。细胞分化及分泌期(P2.5d)和矿化期(P7d),SSUH2均在成釉细胞、成牙本质细胞、牙釉质、上皮根鞘中呈强阳性表达。结论:SSUH2在小鼠牙胚发育中均有表达,且各个时期略有差异,SSUH2可能参与小鼠的牙胚发育。
Objective：To investigate the role of Ssuh2 associated with dentin dyspepsia type I（DD-I）in the period of tooth germ development in mice.Methods：The time of E13.5 d,E14.5 d,E16.5 d,P2.5 d,and P7 dwere determined by HE staining.The expression of SSUH2 was observed by immunohistochemistry.Results：During the bud stage（E13.5 d）,SSUH2 was positively expressed in epithelial cells and mesenchymal cells.During the cup period（E14.5 d）and the early bell stage（E16.5 d）,SSUH2 was strongly positively expressed in the basement membrane,internal and external epithelium,enamel knot,and cervical loop.During the cell differentiation and secretory stage（P2.5 d）and mineralization stage（P7 d）,SSUH2 was strongly expressed in ameloblasts,odontoblasts,enamel,and epithelium root sheath.Conclusion：SSUH2 was expressed during all stages of mouse tooth germ,however,the expression of SSUH2 was slightly different in each period.SSUH2 might involve in the development of tooth germs in mice.
出处
《口腔医学研究》
CAS
北大核心
2018年第1期14-17,共4页
Journal of Oral Science Research
基金
国家自然科学基金资助项目(编号:81470033)
