抗坏血酸碳点通过促进自噬杀伤舌鳞癌细胞

Ascorbic Acid Carbon Dots Kill Tongue Squamous Cell Carcinoma Cell by Promoting Autophagy

目的:研究抗坏血酸碳点(ascorbic acid carbon dots,AACDs)对舌鳞癌细胞系Cal27的杀伤作用及其机制,为碳点在舌鳞癌治疗的应用奠定基础。方法:以Cal27为空白对照组,AACDs组、3-甲基腺苷(3-MA)组及AACDs+3-MA组为实验组,运用MTT法检测细胞增殖活性,吖啶橙(AO)染色法观察细胞自噬情况,流式细胞术检测细胞凋亡情况。结果:MTT结果显示,AACDs能够抑制Cal27的增殖活性,并呈明显的剂量依赖性;AO染色结果显示,AACDs可以促进Cal27发生自噬,其中100 mg/L AACDs引发的自噬水平最高;加入3-MA降低AACDs引发的自噬水平后,细胞增殖活性水平升高,同时细胞凋亡水平降低。结论:AACDs通过促进自噬对Cal27发挥杀伤作用。

Objective：To study the lethal impact and mechanism of ascorbic acid carbon dots（AACDs）on tongue squamous cell carcinoma cell line Cal27.Methods：The blank control group was untreated Cal27,while the groups treated with AACDs,3-methyladenine,and both of them were the experimental groups.MTT assay was used to detect the cell proliferation.Acridine orange staining was used to evaluate the cell autophagy.Flow cytometry was performed to examine cell apoptosis.Results：AACDs suppressed the proliferation of Cal27 on a dose-dependent manner.It also induced the autophagy of Cal27,especially in the 100μg/ml AACDs group.When 3-methyladenine was added to induce the autophagy level,cell proliferation level was improved with the decrease of apoptotic rate.Conclusion：AACDs kill tongue squamous cell carcinoma cells through promoting autophagy.