Twist1调控上皮-间质转化促进舌鳞癌细胞顺铂耐药机制的研究

Study on Twist1 promoting epithelial-mesenchymal transition and drug-resistance in tongue squamous cell carcinoma

目的 :探讨Twist1调控上皮-间质转化(EMT)促进舌鳞状细胞癌细胞顺铂耐药的机制。方法 :在CAL27/CDDP中转染Twist1 siRNA,免疫印迹及免疫荧光检测E-cadherin、Vimentin的表达;免疫印迹检测Twist1、Slug的表达;Transwell及划痕实验检测细胞的侵袭、迁移能力;MTT检测细胞对顺铂的半抑制率。采用SPSS17.0软件包对结果进行单因素方差分析。结果:转染Twist1 siRNA可逆转CAL27/CDDP的EMT表型,E-cadherin表达增强,Vimentin、Twist1及Slug表达降低,细胞的侵袭、迁移能力明显降低,IC_(50)下降41.75%±5.10%(P<0.01)。结论:Twist1可通过调控上皮-间质转化而促进舌鳞癌细胞的顺铂耐药。

URPOSE： The aim of this study was to investigate the mechanism of Twist1 regulating epithelial-mesenchymal transition （EMT） and cisplatin-resistance in tongue squamous carcinoma cells. METHODS： TSCC CDDP resistant cell line CAL27/CDDP was transfected with Twist1 siRNA. Western blot and immunofluorescence staining were used to detect the expression of E-cadherin and Vimentin; Western blot was used to detect the expression of Twist1 and Slug; Transwell assay and scratch test were used to detect the invasion and migration capabilities; MTT was used to analyze the half maximal inhibitory concentration （IC50） values. One-way ANOVA was used to analyze the results with SPSS 17.0 software package. RESULTS： Twist1 siRNA transfection reversed EMT phenotype of CAL27/CDDP. The expression of E-cadherin was up-regulated and the expression of Vimentin, Twist1 and Slug was down-regulated. The motility was significantly decreased. The IC50 of CAL27/CDDP decreased by 41.75%±5.10%（P〈0.01）. CONCLUSIONS： Twist1 promotes EMT and CDDP drug-resistance in TSCC.