胰高血糖素样肽1类似物对2型糖尿病大鼠肾脏功能及超微结构的影响

Effects of GLP-1 analogues on kidney function and kidney ultrastructure in type 2 diabetic rats

目的·观察胰高血糖素样肽1(glucagon-like peptide-1,GLP-1)类似物对2型糖尿病大鼠肾功能及肾脏超微结构的影响。方法·28只SPF级雄性SD大鼠随机分为对照组(正常对照组和糖尿病对照组)和GLP-1类似物艾塞那肽(Exenatide)干预组(正常干预组和糖尿病干预组)。观察Exenatide干预对大鼠糖脂代谢指标、肾功能指标及肾脏超微结构的影响。结果·Exenatide干预组大鼠空腹血糖、空腹胰岛素、胰岛素敏感指数、胰岛素抵抗指数、血脂、24 h尿微量蛋白、血清肌酐、血清尿素氮等指标均显著优于相应对照组。透射电子显微镜观察发现糖尿病对照组大鼠肾小球系膜细胞增生,基膜明显增厚;而糖尿病干预组肾小球系膜细胞增生不明显,基膜轻度增厚。结论·GLP-1类似物对糖尿病大鼠的肾脏具有保护作用,可能与其改善肾脏超微结构有关。

Objective To study the effects of glucagon-like peptide-1 （GLP-1） analogues on kidney function and kidney ultrastructure in type 2 diabetic rats. Methods Twenty-eight SPF level male SD rats were randomly divided into control groups （normal control group and diabetic control group） and GLP-1 analogues Exenatide intervention groups （normal intervention group and diabetic intervention group）. The effects of Exenatide intervention on glucose, lipid metabolism, kidney function and kidney ultrastructure were observed. Results Fasting glucose, fasting insulin, insulin sensitivity index, insulin resistance index, lipid profile, 24-hours microalbuminuria, serum creatinine and blood urea nitrogen of Exenatide intervention group were much better than those of corresponding control groups. Through electron microscopy, glomerular mesangial cell in diabetic control group proliferated, basement membrane thickened, while glomerular mesangial cell in diabetic intervention group did not proliferate, basement membrane mildly thickened. Conclusion. GLP-1 analogues can improve kidney function of diabetic rats. Maybe it is connected with improving kidney ultrastructure.