大鼠C6胶质瘤细胞的基因表达谱

Gene expression profiles of rat C6 glioma cell line

目的:筛选比较大鼠C6胶质瘤细胞与正常星型胶质细胞中的差异表达基因。方法:利用Illumina Hi Seq4000技术对大鼠C6胶质瘤细胞和正常星形胶质细胞进行转录组测序并对测序结果进行功能注释。以|log2 Fold Change|≥1和q值<0.05为标准筛选差异表达基因,并通过GO和KEGG数据库分析其功能和参与的信号通路。结果:较之正常星形胶质细胞,大鼠C6胶质瘤细胞中共筛选得到4363个差异表达基因,其中1603个基因表达上调,2760个基因表达下调;GO富集结果表明:4109个差异表达基因共映射到906个GO term,主要与GTP酶活性正性调节、基因表达正性调节、蛋白结合和钙离子结合等功能相关;KEGG富集结果表明:1535个差异表达基因共参与106条通路,富集最多的是PI3K-Akt信号通路和癌症发生通路。结论:成功获取大鼠C6胶质瘤细胞与正常胶质细胞的基因表达谱,并对其差异基因进行GO和KEGG注释,结果有助于胶质瘤发生发展的研究。

Objective： To screen and lompare the differentially expressed genes between rat C6 glioma cells and normal astrocytes. Methods： The RNA of rat C6 glioma ceils and normal astrocytes were sequenced using Illumina HiSeq4000 and its results were annotated and analyzed with the Kyoto Encyclopedia of Genes and Genomes （ KEGG）, Gene Ontology （GO） database. The logarithm used to identify the differentially expressed genes was ｜ log2 FoldChange｜≥ 1 and q 〈 0.05. Results： A total of 4363 differentially expressed genes were screened from C6 glioma cells, com- pared with normal astrocytes, and 1603 genes were up-regulated and 2760 genes were down-regulated. GO enrichment results showed that 4109 differentially expressed genes were mapped to 906 GO terms, which were mainly related to the positive regulation of GTPase activity, positive regulation of gene expression, protein binding and calcium ion binding. KEGG enrichment results showed that 1535 differentially expressed genes were involved in 106 patways and the most relevant pathways are PI3K-Akt and cancer-related. Conclusion： The gene expression profiles in rat C6 glioma cells and normal astrocytes were obtained successfully, and differentially expressed genes were annotated by GO and KEGG, which may contribute to a better understanding of glioblastoma pathophysiology and disease stratification.