Clusterin与乳腺癌淋巴道转移的相关性研究

Correlation between Clusterin and lymphatic metastasis of breast cancer

目的考察Clusterin在乳腺癌中的表达情况,以及Clusterin与淋巴道转移的相关性。方法选取我院2015年6月~2016年6月经病理证实的乳腺癌患者55例,31例乳腺癌良性病例患者,21例癌旁组织3cm作为对照组。免疫组化SP法检测Clusterin和D2-40蛋白表达水平,计算微淋巴管密度。结果 Clusterin主要表达于细胞质;乳腺癌组织中Clusterin阳性率显著高于乳腺良性组织和癌旁组织(P<0.05);乳腺癌组织中MLVD显著高于乳腺良性组织和癌旁组织(P<0.05);出现淋巴结转移的患者Clusterin阳性率高于无淋巴结转移的患者,数据差异具有统计学意义(χ~2=7.882,P<0.05)。结论乳腺癌患者Clusterin蛋白表达水平上调,出现淋巴结转移的乳腺癌患者Clusterin表达阳性率上升。

Objective To investigate the expression of Clusterin in breast cancer,correlation between Clusterin and lymphatic metastasis. Methods From June 2015 to June 2016, 55 cases of breast cancer patients , 31 cases of benign breast cancer patients, 21 cases of 3cm adjacent cancer tissue as control group. The expression levels of Clusterin and D2-40 were detected by immnnohistochemical SP method, and the density of lymphatic vessels was calculated. Results Clusterin was mainly expressed in the cytoplasm; The positive rate of Clusterin in breast cancer was significantly higher than that in benign breast tissue andparacancerous tissue（P〈0.05）; MLVD in breast cancer was significantly higher than that in benign breast tissue and paracancerous tissue（P〈 0.05）; The positive rate of Clusterin in patients with lymph node metastasis was higher than that in patients without lymph node metastasis（X2=7.882, P 〈 0.05）. Conclusion The expression of Clusterin protein in breast cancer patients was up- regulated.The positive rate of Clusterin expression in breast cancer patients with lymph node metastasis was increased.