基于UPLC-Q-Orbitrap MS/MS研究人参皂苷在发酵过程中的生物转化

Biotransformation of Ginsenosides in Fermented Ginseng Using UPLC-Q-Orbitrap MS/MS

应用超高效液相色谱-四极杆-静电场轨道阱串联质谱(UPLC-Q-Orbitrap MS/MS)联用技术分析鉴别人参发酵前后的皂苷类化合物,研究了整体人参皂苷在自然发酵过程中的生物转化规律.通过鉴定45种人参皂苷,比较了生晒参和发酵人参中人参皂苷种类及含量的差异,在发酵人参中检测到20(S)-Rg_3和20(R)-Rg_3,20(S)-Rh_1和20(R)-Rh_1等异构体人参皂苷,其中10余种人参皂苷在自然人参状态下不存在或未被检出.本研究对于人参发酵前后的化学成分变化进行了高通量的精准分析,对同类天然产物发酵研究具有借鉴意义,可指导人参炮制品生晒参和发酵人参的安全有效使用.

This work aims to analyze and compare the ginsenosides contents from white ginseng and fermented black ginseng（FBG）using UPLC-Q-Orbitrap MS/MS（ultra performance liquid chromatography coupled with quadrupole orbitrap tandem mass spectrometry）.FBG was processed by spontaneous short-term fermentation,along with the biotransformation of ginsenosides.The samples were separated by liquid chromatography using a Thermo Syncronis C_（18）column at 30℃.Moreover,0.1%（volume fraction）formic acid and acetonitrile were used as mobile phases A and B,respectively.The flow rate was 0.2 m L/min,and the injected sample volume was 0.5μL.By the use of the UPLC-Q-Orbitrap MS/MS,52 kinds of ginsenosides were identified.The contents of main ginsenosides,especially in malonylated ginsenosides,were decreased,because of hydrolyzed.The method of spontaneous short-term fermentationdid doesn’t require any additives,and provides the theoretical basis for spontaneous short-term fermentprocessed ginseng.