摘要
应用超高效液相色谱-四极杆-静电场轨道阱串联质谱(UPLC-Q-Orbitrap MS/MS)联用技术分析鉴别人参发酵前后的皂苷类化合物,研究了整体人参皂苷在自然发酵过程中的生物转化规律.通过鉴定45种人参皂苷,比较了生晒参和发酵人参中人参皂苷种类及含量的差异,在发酵人参中检测到20(S)-Rg_3和20(R)-Rg_3,20(S)-Rh_1和20(R)-Rh_1等异构体人参皂苷,其中10余种人参皂苷在自然人参状态下不存在或未被检出.本研究对于人参发酵前后的化学成分变化进行了高通量的精准分析,对同类天然产物发酵研究具有借鉴意义,可指导人参炮制品生晒参和发酵人参的安全有效使用.
This work aims to analyze and compare the ginsenosides contents from white ginseng and fermented black ginseng(FBG)using UPLC-Q-Orbitrap MS/MS(ultra performance liquid chromatography coupled with quadrupole orbitrap tandem mass spectrometry).FBG was processed by spontaneous short-term fermentation,along with the biotransformation of ginsenosides.The samples were separated by liquid chromatography using a Thermo Syncronis C_(18)column at 30℃.Moreover,0.1%(volume fraction)formic acid and acetonitrile were used as mobile phases A and B,respectively.The flow rate was 0.2 m L/min,and the injected sample volume was 0.5μL.By the use of the UPLC-Q-Orbitrap MS/MS,52 kinds of ginsenosides were identified.The contents of main ginsenosides,especially in malonylated ginsenosides,were decreased,because of hydrolyzed.The method of spontaneous short-term fermentationdid doesn’t require any additives,and provides the theoretical basis for spontaneous short-term fermentprocessed ginseng.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2018年第2期219-225,共7页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:3140040275)
吉林省科学技术发展计划项目(批准号:20160101152JC)资助~~
关键词
发酵
人参皂苷
生物转化
液相色谱-质谱联用
Fermentation
Ginsenoside
Biotransformation
Liquid chromatography-mass spectrometer