胰腺导管腺癌中腺苷酸激酶4的表达及临床意义

Expression of AK4 in pancreatic ductal carcinoma and its clinical significance

目的:探讨腺苷酸激酶4(AK4)在胰腺导管腺癌组织中的表达及其临床意义。方法:回顾性分析76例接受胰腺癌根治手术治疗的胰腺癌患者的临床与随访资料。采用免疫组织化学法检测76例胰腺癌组织和对应的癌旁组织中AK4的表达,分析其与临床病理学特征及预后的关系。结果:AK4的阳性表达主要在细胞浆内,呈棕黄色颗粒。AK4在胰腺癌和癌旁组织中的阳性表达率分别为78.9%(60/76)和38.2%(29/76),前者表达率显著高于后者,差异有统计学意义(P<0.05)。AK4的异常表达与肿瘤分期、淋巴结转移、神经受侵、脉管内瘤栓相关(P<0.05)。生存分析提示在胰腺导管腺癌中AK4高表达的患者生存时间明显短于低表达的患者(P<0.05)。结论:胰腺导管腺癌组织中存在AK4表达水平的上调,AK4的表达可能提示胰腺癌患者分期较晚和预后不良。

Objective： To explore the expression of AK4 in pancreatic ductal carcinoma and its clinical significance. Methods： The clinical and follow-up data of 76 patients with pancreatic ductal carcinoma who underwent radical surgery were retrospectively analyzed.Immunohistochemistry was used to detect the expression of AK4 in 76 cases of pancreatic cancer and its adjacent tissues, and the relationship of AK4 with clinicopathological features and prognosis was analyzed. Results： The positive expression of AK4 mainly was in the cytoplasm, presenting brownish yellow granules. AK4 in pancreatic ductal adenocarcinoma tissues or paracancerous tissue were 78.9%（60/76） and 38.2%（29/76）, and the positive rate in pancreatic ductal adenocarcinoma tissues was significantly higher than that in paracancerous tissue. The difference was statistically significant（P〈0.05）. The abnormal expression of AK4 was correlated with the tumor stage, lymph node metastasis, neural invasion, and the vascular tumor embolus（P〈0.05）. Survival analysis suggested that the survival time of patients with AK4 high expression in pancreatic duct adenocarcinoma was significantly shorter than those with low expression（P〈0.05）.Conclusion： Up-regulation of AK4 expression in pancreatic ductal carcinoma tissues, is associated with the tumor stage, lymph node metastasis, nerve invasion, and the vascular tumor thrombus, which might indicate the late stage and poor prognosis for the patients with pancreatic ductal carcinoma.