鹿芪方对高血压合并心力衰竭大鼠血压、血管紧张素-醛固酮和心肌胶原纤维的影响

Effect of Luqi formula on blood pressure,angiotensin-aldosterone and myocardial collagen fibers in rats with hypertension complicated with heart failure

[目的]研究鹿芪方对高血压合并心力衰竭大鼠血压、血管紧张素Ⅰ(AngⅠ)、血管紧张素Ⅱ(AngⅡ)、醛固酮(ALD)和心肌胶原纤维的影响。[方法]采用腹主动脉缩窄术致高血压合并心力衰竭大鼠模型,造模成功后,将大鼠随机分为模型组,对照组,假手术组,鹿芪方高、中、低剂量组。给药8周后测量大鼠血压。应用双抗体夹心酶联免疫吸附法测定大鼠AngⅠ、AngⅡ、ALD水平。采用苦味酸天狼星红染色观察大鼠心肌胶原纤维改变。[结果]与模型组相比,鹿芪方各剂量组能有效降低心力衰竭大鼠的收缩压(P<0.05),其中高、中剂量组优于低剂量组(P<0.05)。与模型组比较,鹿芪方各剂量组能有效降低AngⅠ、AngⅡ及ALD(P<0.05),在降低ALD方面,鹿芪方高剂量组明显优于低剂量组(P<0.01)。通过对大鼠心肌天狼星红染色发现,鹿芪方各剂量组较模型组而言,能显著减少心肌胶原纤维含量,改善心肌间质形态,鹿芪方高剂量组要优于鹿芪方中、低剂量组。[结论]鹿芪方能降低心力衰竭大鼠的血压,调节神经内分泌的激活,减轻心肌纤维化,改善心室重构从而减轻心肌受损。

[Objective] To study the effect of Luqi formula on blood pressure,angiotensin I,angiotensin II,aldosterone and myocardial collagen fibers in rats with hypertension complicated with heart failure.[Methods] The rat models of hypertension complicated with heart failure was induced by abdominal aorta constriction.After successful modeling,the rats were randomly divided into model group,the control group,the sham operation group,the high,middle and low dose of Luqi formula groups.After 8 weeks of administration,measurement of blood pressure in rats.Determination of angiotensin I（Ang I）,angiotensin II（Ang II） and aldosterone（ALD） levels in rats with ELISA double antibody sandwich enzyme.Picrosirius red staining was used to observe the changes of myocardial collagen fibers in rats.[Results] Compared with the model group,each dose of Luqi formula can effectively reduce systolic blood pressure in rats with heart failure（P0.05）.The high and middle dose gruops were better than the low dose group（P0.05）.Compared with the model group,each dose group of Luqi formula can effectively reduce Ang I,Ang II and ALD（P0.05）.The high dose group of Luqi formula was obviously better than the low dose group in reducing ALD（P 0.01）.Through the picrosirius red staining of rats＇ myocardium,each dose group of Luqi formula compared with model group can significantly reduce the myocardial collagen content,improve myocardial interstitial morphology.The high dose group of Luqi formula was better than the middle and low groups.[Conolusion] Luqi formula can reduce blood pressure of heart failure rats,regulate neuroendocrine activation,reduce myocardial fibrosis,improve ventricular remodeling and reduce myocardial damage.