UPLC/ESI-Q-TOF MS法分析远志中的化学成分

Analysis of chemical constituents of Radix Polygalae by UPLC/ESI-Q-TOF MS

[目的]阐明远志中的化学成分。[方法]采用超高效液相色谱-电喷雾离子化-四级杆飞行时间串联质谱(UPLC/ESI-Q-TOF MS)法分析远志中的化学成分,采用Masslynx V 4.1软件,通过分析准分子离子和二级碎片离子信息,并进一步根据标准品的色谱保留时间和质谱裂解规律,鉴定远志中的化学成分。[结果]鉴定了远志中29个化学成分,主要为口山酮、低聚糖酯、皂苷类成分。[结论]总结归纳了远志药材中主要化学成分的质谱裂解规律,阐明了远志中的主要化学成分。

[Objective] To elucidate the chemical constituents of Radix Polygalae（RP）.[Methods] Ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry（UPLC/ESI-Q-TOF） controlled by Masslynx V 4.1 software was employed for identification of the components in RP.By integrating the information of quasi-molecular and fragment ion,and the retention time and fragment pattern of standards,compounds of RP were identified.[Results] The 29 compounds including xanthones,oligosaccharide esters and saponins of RP were identified.[Conclusion] In this study,the fragment pattern of major compounds in RP was summarized,which provided a basis for further elucidation of chemical substances in RP.