负压吸引装置抽取大鼠脑脊液方法的改良

A modified suction device with negative pressure for cerebrospinal fluid collection in rats

目的在负压吸引装置法抽取大鼠脑脊液的基础上进行改良,进一步提高大鼠脑脊液收集的成功率。方法通过增加流量调节器控制压负强度、限定脑脊液的每次抽取量对原装置进行改良;36只雄性SD大鼠随机分为3组,分别用经皮延髓池穿刺法、负压吸引装置法、改良负压吸引装置法抽取脑脊液,用Kruskal-Wallis秩和检验评价3种方法的差异。结果改良负压吸引装置法能有效减少出血,取得的脑脊液的质量高于负压吸引装置法(P<0.01)及经皮穿刺延髓池穿刺法(P<0.001),抽取合格样本的成功率为75%。结论改良负压吸引装置制作方便,操作简单,损伤小,不易出血,可明显提高大鼠脑脊液采集的成功率。

Objective To improve the success rate of cerebrospinal fluid（ CSF） collection in rats using a modified suction device with negative pressure. Methods A flow regulator was added to the original apparatus to adjust the intensity of negative pressure and to limit the volume of CSF collected each time. A total of 36 male Sprague-Dawley（ SD） rats were randomly divided into 3 groups,and the CSF was collected in 3 ways： drawing from the cerebellomedullary cistern via percutaneous puncture,using a suction device with negative pressure and using a modified device. KruskalWallis test was used to evaluate the differences in result between these three methods. Results The modified suction device with negative pressure effectively reduced bleeding. The quality of CSF collected with it was 75% of 12 rats,significantly higher than that obtained with the original device（ 33. 3%,P〈0. 01） or drawn from the cerebellomedullary cistern via percutaneous puncture（ 0%,P〈0. 001）. The success rate of qualified sample collection was highly superior to those of previously reported CSF collection methods. Conclusions Our modified suction device for CSF collection is simple and user-friendly,with minimal damage and little bleeding to rats. It can obviously improve the success rate of CSF collection in rats.