响应面试验优化诺丽果黄酮微波辅助提取工艺

Optimization of microwave-assisted extraction process of flavonoids from Noni by response surface experiments

以诺丽果为原料,通过单因素及响应面试验对诺丽果中黄酮类化合物的微波辅助提取工艺进行优化,结果表明,黄酮类化合物最佳提取条件为乙醇体积分数50%,液料比50∶1(mL∶g),微波辅助提取时间3 min,微波功率300 W,在该条件下获得的黄酮提取率的平均值为25.98 mg/g。通过试验得出,当提取液质量浓度为8.00 mg/mL时对DPPH自由基、·OH去除率最高,分别为92.8%、82.2%,证明其具有较好的体外抗氧化能力。

Using Noni as raw material, the microwave-assisted extraction process of flavonoids compounds from Noni was optimized by single factor and response surface experiments. The results showed that the optimum extraction conditions of flavonoids compounds were ethanol concentration50%, liquid-solid ratio 50∶1(ml∶g), microwave-assisted extraction time 3 min and microwave power 300 W. Under the conditions, the average extraction rate of flavonoids compound was 25.98 mg/g. Extracting solution 8.00 mg/ml had the highest scavenging rate on DPPH free radical and·OH(92.8%and 82.2%, respectively), which indicated that it had good antioxidant ability in vitro.