摘要
目的探讨针刺内关、水沟抑制脑缺血再灌注大鼠大脑皮层Caspase-3、PARP-1 mRNA和蛋白表达的影响。方法 40只雄性SD大鼠,随机分为正常组、假手术组、模型组、针刺组。采用改进的Longa线栓法制备大脑中动脉梗塞模型。参考Bederson6级5分法进行神经功能评分,运用Real-time PCR、Western Blot检测脑缺血后24 h大鼠大脑皮层Caspase-3、PARP-1的mRNA及蛋白表达量变化。结果与模型组相比,针刺组可改善缺血再灌注大鼠神经功能缺损症状(P<0.01);针刺可下调Caspase-3、PARP-1的mRNA及蛋白表达,与模型组比较差异具有统计学意义(P<0.05,P<0.01;P<0.05,P<0.05)。结论针刺内关、水沟可下调脑缺血再灌注大鼠大脑皮层Caspase-3、PARP-1的mRNA及蛋白表达。
Objective To investigate the inhibiting effect of acupuncture at points Neiguan(PC6) and Shuigou(GV26) on caspase-3, and PARP-1 mRNA and protein expressions in the cerebral cortex in rats with cerebral ischemia-reperfusion. Method Forty male SD rats were randomized to normal, sham operation, model and acupuncture groups. A model of middle cerebral artery infarction was made by modified Longa thread occlusion. Neurological function was scored using a 6-grade 5-point Bederson’s scale. Caspase-3, and PARP-1 mRNA and protein expressions in rat cerebral cortex were determined using real-time PCR and Western Blot at 24 hrs after cerebral ischemia. Result Neurological deficit was reduced in the acupuncture group of rats with cerebral ischemia-reperfusion compared with the model group (P〈0.01). Acupuncture could down-regulate caspase-3, and PARP-1 mRNA and protein expressions and there was a statistically significant difference compared with the model group (P〈0.05, P〈0.01; P〈0.05, P〈0.05). Conclusion Acupuncture at points Neiguan and Shuigou can down-regulate caspase-3, and PARP-1 mRNA and protein expressions in the cerebral cortex in rats with cerebral ischemia-reperfusion.
出处
《上海针灸杂志》
2018年第2期234-238,共5页
Shanghai Journal of Acupuncture and Moxibustion
基金
国家自然科学基金面上项目(81674067)
浙江省自然科学基金一般项目(LY17H270009)