摘要
目的评价脊髓caspase-1在大鼠切口痛中的作用。方法取鞘内置管成功的成年雄性SD大鼠18只,体重250~280 g,采用随机数字表法分为3组(n=6):切口痛组(I组)、切口痛+二甲基亚砜组(ID组)和切口痛+caspase-1抑制剂Ac-YVAD-CMK组(IA组)。于切口痛模型制备前10 min,I组鞘内注射0.9%生理盐水20 μl;ID组鞘内注射二甲基亚砜20 μl,然后用0.9%生理盐水10 μl冲洗导管;IA组鞘内注射Ac-YVAD-CMK 1 nmol/μl(溶于20 μl二甲基亚砜中),然后用0.9%生理盐水10 μl冲洗导管。分别于鞘内置管前2 h(T0)、鞘内置管后3 d(T1)、模型制备后2、6、24和48 h(T2-5)时测定术侧足机械缩足反应阈。于T5时痛阈测定结束后,处死大鼠,取脊髓腰膨大,采用Western blot法检测caspase-1(p20)表达,采用ELISA法检测IL-1β含量。结果与T0比较,I组和ID组T2-5机械缩足反应阈降低(P〈0.05),IA组T1-5机械缩足反应阈差异无统计学意义(P〉0.05);与I组和ID组比较,IA组T2-5机械缩足反应阈升高,脊髓caspase-1(p20)表达水平和IL-1β含量降低(P〈0.05);I组和ID组各时点机械缩足反应阈、脊髓caspase-1(p20)表达和IL-1β含量比较差异无统计学意义(P〉0.05)。结论脊髓caspase-1的活化可促进IL-1β的释放,从而参与大鼠切口痛的病理生理过程。
Objective To evaluate the role of caspase-1 in the spinal cord in a rat model of incisional pain.Methods Eighteen adult male Sprague-Dawley rats, weighing 250-280 g, in which intrathecal catheters were successfully implanted, were divided into 3 groups(n=6 each)using a random number table: incision pain group(group I), incision pain plus dimethyl sulfoxide group(group ID)and incision pain plus caspase-1 inhibitor(Ac-YVAD-CMK)group(group IA). At 10 min before establishment of the model, 0.9% normal saline 20 μl was intrathecally injected in group I, dimethyl sulfoxide 20 μl was intrathecally injected and then the catheter was washed with 0.9% normal saline 10 μl in group ID, and Ac-YVAD-CMK 1 nmol/μl(dissolved in 20 μl dimethyl sulfoxide)and then the catheter was washed with 0.9% normal saline 10 μl in group IA.The mechanical paw withdrawal threshold(MWT)of the ipsilateral hind paw was measured at 2 h before intrathecal catheterization(T0), 3 days after intrathecal catheterization(T1)and 2, 6, 24 and 48 h after establishment of model(T2-5). The rats were sacrificed after the last measurement of pain threshold at T5, and lumbar enlargement segments of the spinal cord were removed for detection of caspase-1(p20)expression and interleukin-1beta(IL-1β)content by Western blot and enzyme-linked immunosorbent assay, respectively.Results Compared with the baseline at T0, the MWT was significantly decreased at T2-5 in I and ID groups(P〈0.05), and no significant change was found in MWT at T1-5 in group IA(P〉0.05). Compared with I and ID groups, the MWT at T2-5 was significantly increased at T2-5, and the caspase-1(p20)protein expression and IL-1β content were decreased in group IA(P〈0.05). There was no significant difference in MWT, expression of caspase-1(p20)protein or IL-1β content between group I and group ID(P〉0.05).Conclusion The activation of caspase-1 in the spinal cord can promote the release of IL-1β and thus is involved in the incision pain in rats.
作者
农小连
付艾平
蓝雨雁
Nong Xiaolian;Fu Aiping;Lan Yuyan(Department of Anesthesiology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China)
出处
《中华麻醉学杂志》
CSCD
北大核心
2017年第12期1466-1468,共3页
Chinese Journal of Anesthesiology
基金
广西自然科学基金项目(2011GXNSFA018222)