THBS2在宫颈癌中的表达及功能研究

The expression and function of Thrombospondin-2 in cervical cancer

目的:探讨THBS2在宫颈癌发生发展中的表达水平变化以及THBS2对细胞增殖、迁移和侵袭能力的作用。方法:免疫组化和Western blot法检测THBS2在宫颈病变组织及细胞系中的表达水平。选取THBS2表达水平较低的宫颈癌细胞系Si Ha和ME180,通过THBS2过表达质粒上调THBS2水平。选取宫颈上皮永生化细胞株H8和ECT-1,通过转染THBS2的干扰片段siRNA下调THBS2表达。Western blot法检测THBS2表达,CCK8细胞增殖试验、划痕试验和Transwell侵袭试验观察THBS2对细胞的增殖、迁移和侵袭能力的影响。结果:THBS2在宫颈癌发生发展过程中表达水平下降(P<0.001);宫颈癌细胞系Si Ha和ME180中THBS2表达水平较HPV永生化宫颈上皮细胞H8和ECT-1明显降低(P<0.05)。上调宫颈癌细胞THBS2表达,Si Ha和ME180细胞的增殖(P<0.05)、迁移(P<0.005)和侵袭能力(P<0.001)明显被抑制;下调宫颈上皮永生化细胞THBS2表达,H8和ECT-1细胞的增殖(P<0.05)、迁移(P<0.005)和侵袭能力(P<0.001)显著增强。结论:THBS2在宫颈癌发生发展过程中可能充当"抑癌因子"。

Objective： To investigate the expression of Thrombospondin-2（ THBS2） and its effect on proliferation,migration and invasion during the development of cervical cancer.Methods： The expression of THBS2 in tissue or cell lines was detected by immunohistochemistry and Western blot. Expression plasmid was used to increase the level of THBS2 in cervical cancer cell lines-Si Ha and ME180,and siRNA was used to decrease the expression of THBS2 in H8 and ECT-1 cells. The results were verified using Western blot. CCK8 assay,wound healing and Transwell invasion assay were performed to estimate the effect of THBS2 on proliferation,migration and invasion in cervical cancer cell lines.Results： THBS2 was down-regulated during the development of cervical cancer（ P〈0.001）. And THBS2 in cervical cancer cell lines Si Ha and ME180 was significantly lower than those of H8 and Ect-1（ P〈0.05）.The proliferation（ P〈0.05）,migration（ P 〈 0. 005） and invasion（ P 〈 0. 001） effects were inhibited in Si Ha and ME180 cell lines when THBS2 was overexpressed.The proliferation（ P 〈 0.05）,migration（ P 〈0.005） and invasion（ P 〈 0.001） effects were promoted in H8 and ECT-1 cells when THBS2 was suppressed.Conclusion： THBS2 plays a role as ’cancer suppressive factor’ during the development of cervical cancer and thus may be a potential therapeutic target for treating cervical cancer.