性别对两种溃疡性结肠炎模型的影响

The Effect of Gender on Two Ulcerative Colitis Models Induced by OXZ and TNBS Individually in Mice

目的：分别建立由噁唑酮（oxazolone,OXZ）及2,4,6-三硝基苯磺酸（2,4,6-trinitrobenzene sulfonic acid,TNBS）诱导的小鼠溃疡性结肠炎模型,并探讨性别因素对小鼠模型的影响。方法：30只健康成年昆明种小鼠,雌雄各半,随机分为6组,正常雌性对照组（A组,n=5）,正常雄性对照组（B组,n=5）,OXZ雌性模型组（C组,n=5）,OXZ雄性模型组（D组,n=5）,TNBS雌性模型组（E组,n=5）和TNBS雄性模型组（F组,n=5）。OXZ模型组（n=10）给予皮肤涂抹2.4%OXZ（无水乙醇溶剂）0.2 m L 2 d,5 d后以0.8%OXZ（40%乙醇溶剂）0.1 m L灌肠;TNBS模型组（n=10）于d 1及d 6给予1.8 mg TNBS（40%乙醇溶剂）灌肠,灌肠之前均进行禁食不禁水24 h处理,正常组不作处理。造模24 h之后记录小鼠精神状态、毛色、进食、大便情况并检测小鼠疾病活动指数（disease activity index,DAI）值。造模后d 2处死,分离结肠,测定小鼠结肠组织大体评分（colon macroscopic damage index,CMDI）以及结肠湿重指数（colon index,CI）,并取病变部位检测结肠组织病理学评分（histopathological score,HPS）以及白细胞介素-4（interleukin 4,IL-4）、肿瘤坏死因子-α（tumor necrosis factor-α,TNF-α）的水平。结果：两种溃疡性结肠炎小鼠模型诱导成功,OXZ模型组IL-4及TNF-α水平与正常组差异均有显著性,但TNBS模型组差异较小。除OXZ雌性组结肠指数与正常组没有显著差异外,模型组小鼠各项指标与正常组差异均有显著性。结论：性别对于小鼠溃疡性结肠炎模型的诱导有着重要的影响。

Objective：To establish two ulcerative colitis models in mice induced by oxazolone（OXZ） and 2,4,6-trinitrobenzene sulfonic acid（TNBS） respectively. To explore the influence of gender on mouse models. Methods：30 KM healthy grown mice half male and half female were randomly divided into 6 groups which include group A（normal female group,n=5）,group B（normal male group,n=5）,group C（OXZ female model group,n=5）,group D（OXZ male model group,n=5）,group E（TNBS female model group,n=5）,group F（TNBS male model group,n=5）. Two OXZ model groups were coated on the skins with 2.4% OXZ solution（100% ethanol solutions） 0.2 m L as skin sensitization in 2 days. At the sixth day,the two model groups were given the enema（0.1 m L 0.8% OXZ dissolved in 40% ethanol）. The two TNBS model groups were administered with enema（1.8 mg TNBS dissolved in 40% ethanol） at the day 1 and day 6.Fast cannot help but water all model groups mice for 24 hours before the operation of enema. Normal group was not dealt with anything. The mental state,hair color,eating situation and the soil of mice were recorded and the activity index disease（DAI） values were evaluated one day after the molding. Then the mice were sacrificed and the colon was isolated out. The colon macroscopic damage index（CMDI） and the colon index（CI） were recorded. The level of the interleukin 4 and the tumor necrosis factor-α of the lesion site were detected. Results：Two kinds of mice ulcerative colitis model were induced successfully. The IL-4 and TNF-α levels of OXZ female model groups were significantly different from the OXZ male model group,but the TNBS model groups were not significant. The all indexes of model groups were significantly different from the normal group,except the CI of OXZ female group. Conclusion：Both OXZ and TNBS could induce the ulcerative colitis model in mice which pathological characteristics were different. The gender factors have important influence on the induction of the ulcerative colitis model in mice.