金花茶叶中1个新的鞣花酸类化合物

A new ellagic acid from leaves of Camellia chrysantha

目的研究金花茶Camellia chrysantha叶的化学成分。方法采用硅胶柱色谱、凝胶柱色谱、重结晶、制备型高效液相色谱法等现代分离方法和技术对其化学成分进行分离纯化,并根据理化性质和波谱数据进行结构鉴定。结果从金花茶叶中分离得到了10个化合物,经鉴定分别为3,4-methylenedioxy-3′-O-methyl-4′-O-(6′-O-acetyl-glucoside)ellagic acid(1)、齐墩果酸(2)、α-菠菜甾醇-3-O-β-D-葡萄糖(3)、正三十二烷醇(4)、okicamelliaside(5)、6-羟基山柰酚-3-O-β-D-葡萄糖苷(6)、3,4-O,O-次甲基鞣花酸(7)、鞣花酸-4-O-β-D-吡喃葡萄糖苷(8)、3,4-亚甲二氧基-3′-O-甲基-4′-O-葡萄糖苷鞣花酸(9)、p-(羟甲基)苯酚-5-O-β-D-葡萄糖吡喃糖基(1→2)-β-D-(4-O-对-香豆酰)-吡喃葡萄糖苷(10)。结论化合物1为新的鞣花酸类化合物,命名为金花茶素A,化合物4~10均为首次从该植物中分离得到。

Objective To investigate the chemical constituents in the leaves of Camellia chrysantha. Methods The chemical constituents were separated and purified by silica gel, Sephadex LH-20 column chromatography, recrystallization, preparative HPLC and so on. Their structures were determined by physical constants and spectral analyses. Results Ten compounds were isolated and identified as 3,4-methylenedioxy-3′-O-methyl-4′-O-（6′-O-acetyl-glucoside） ellagic acid（1）, oleanolic acid（2）, α-spinasteryl-3-O-β-D-glycoside（3）, n-dotriacontanol（4）, okicamelliaside（5）, 6-hydroxy-kaempferol-3-O-β-D-glucopyranoside（6）, 3,4-O,Omethylidyne-ellagic acid（7）, ellagic acid-4-O-β-D-glucopyranoside（8）, 3,4-methylenedioxy-3＇-O-methyl-4＇-O-glucoside ellagic acid（9）, and p-（hydroxymethyl） phenol 5-O-β-D-glucopyranosyl（1→2）-β-D-（4-O-p-coumaryl）-glucopyranoside（10）. ConclusionCompound 1 is a new ellagic acid named camellia A. Compounds 4—10 are obtained from this plant for the first time.