KLF2/RelA比例失衡与哮喘患者中性粒细胞凋亡的关系研究

The relationship between KLF2/RelA imbalance and neutrophil apoptosis in asthmatic patients

目的明确支气管哮喘患者外周血中性粒细胞内是否存在KLF2/RelA比例失衡,并探讨KLF2/RelA比例失衡与中性粒细胞凋亡的关系。方法收集2011年4月至2012年4月在湖南省人民医院及长沙市第三人民医院住院的39例哮喘急性发作期患者,其中轻度哮喘组13例,中度哮喘组17例,重度哮喘组9例;另收集15例健康人为对照组。抽取受试者外周静脉血,分离纯化中性粒细胞,通过流式细胞术检测中性粒细胞凋亡率,Western blot检测KLF2和RelA在中性粒细胞内的表达。采用Pearson相关检验分析KLF2/RelA比值与中性粒细胞凋亡之间的关系。结果轻、中、重度哮喘组中性粒细胞凋亡率较对照组降低[(4.45±0.76)%、(2.10±0.25)%、(1.81±0.67)%比(5.36±0.57)%,均P<0.01],中、重度哮喘组中性粒细胞凋亡率较轻度哮喘组降低(P<0.01),但中度哮喘组与重度哮喘组比较差异无统计学意义(P>0.05)。轻、中、重度哮喘组中性粒细胞内KLF2/RelA比值较对照组显著降低(0.667±0.351、0.384±0.203、0.536±0.293比4.038±2.011,均P<0.01),轻、中、重度哮喘组间两两比较,差异无统计学意义(均P>0.05)。中性粒细胞凋亡率与KLF2/RelA比值呈正相关(r=0.592 0,P<0.000 1)。结论哮喘患者外周血中性粒细胞内存在KLF2/RelA比例失衡,并且KLF2/RelA比例失衡可能是导致哮喘患者外周血中性粒细胞凋亡减少的机制。

ObjectiveTo determine whether there is an imbalance of KLF2/RelA in peripheral blood neutrophils in patients with bronchial asthma, and explore the relationship between KLF2/RelA imbalance and neutrophil apoptosis.MethodsFrom April 2011 to April 2012, a total of 39 patients with acute attack of asthma in Hunan People＇s Hospital and Third People＇s Hospital of Changsha were enrolled, with 13 cases in mild asthma group, 17 cases in moderate asthma group, and 9 cases in severe asthma group. Fifteen healthy subjects were recruited as control group. Peripheral blood were collected from all subjects followed by separation of neutrophils. The apoptosis of neutrophils were measured by flow cytometry. The expression of KLF2 and RelA were detected by Western blot. The relationship between the ratio of KLF2/RelA and neutrophil apoptosis rate was analyzed by Pearson correlation test.ResultsNeutrophil apoptosis rates in the mild, moderate and severe asthma groups [（4.45±0.76）%, （2.10±0.25）%, （1.81±0.67）%, repectively] were lower than that in the healthy control group [（5.36±0.57）%, all P〈0.01]. The apoptosis rates of neutrophils in the moderate and severe asthma groups were lower than that in the mild asthma group （bothP〈0.01）, but there was no significant difference between the moderate asthma group and the severe asthma group （P〉0.05）. The ratios of neutrophil KLF2/RelA in the mild, moderate and severe asthma groups were lower than that in the normal control group （0.667±0.351, 0.384±0.203, 0.536±0.293vs. 4.038±2.011, all P〈0.01）. There was no significant difference between the groups of mild, moderate and severe asthma （P〉0.05）. The neutrophil apoptosis rate was positively correlated with the percentage of neutrophil KLF2/RelA （r=0.592 0, P〈0.000 1）.ConclusionThere is an imbalance of KLF2/RelA in peripheral blood neutrophils in patients with bronchial asthma, and the imbalance of KLF2/RelA may be the mechanism of apoptosis of peripheral blood neutrophils.