辽宁绒山羊多胎基因ESR靶标microRNA的筛选

Screening of the ESR target microRNA in Liaoning cashmere goats

本文通过对辽宁绒山羊组织DNA的提取、DNA浓度和纯度的检测、基因目的片段的扩张、序列测定与SNP定位等试验,得到了micro RNA的分离与靶标结合的特点,筛选得到的micro RNA的特点等。试验结果表明,利用在线软件mi RBase查找到与羊相关的micro RNA有137个相关,用Segal Lab of Computational Biology软件将ESR基因与羊的micro RNA结合情况分析后,发现突变前结合能够与靶基因结合的micro RNA有2个,突变后结合的micro RNA也是2个。筛选得到的RNA测序后通过基因序列与波峰的比对发现,在ESR基因3′-UTR区发现了1个差异位点,648 bp位点A/T的突变,旨在为今后羊的多态性的研究提供科学的理论依据。

The paper extract Liaoning cashmere goats DNA of muscle tissue, DNA concentration and purity testing, the purpose of the expansion of gene fragments, sequencing and SNP locate these experiments, micro RNA got separated and target binding characteristics, features screened micro RNA like. Experiment results show that the use of online software to find goats micro RNA, goats target micro RNA library contains 137 related micro RNA, with mi RBASE software ESR micro RNA genes and goats combined analysis found that after the first two mutations bound micro RNA, after two mutations bound micro RNA. After screened by sequencing RNA gene sequence alignment with crest found in ESR gene 3＇-UTR region found a difference between sites, 648 bp locus A/T mutation. This article through to the ESR gene 3＇-UTR region mutation and the combination of micro RNA analysis,screening out the relevant micro RNA, aimed at the future of the study of the polymorphism of cashmere goats to provide a scientific basis for the theory, provide a theoretical basis for further study of animal micro RNA.