摘要
目的研究11味中药提取物对脂多糖(LPS)诱导巨噬细胞RAW264.7产生一氧化氮(NO)的抑制作用。方法使用MTT法测试样品对RAW264.7的细胞毒作用,Griess法测定样品对LPS刺激RAW264.7细胞产生NO的含量,N-单甲基-L-精氨酸单乙酸酯(L-NMMA)为阳性对照。结果 11味中药甲醇提取物均具有一定的抑制RAW264.7细胞生成NO的作用,其中秦艽、五加皮、独活、雷公藤、仙鹤草提取物的抑制作用最强(IC50在4.3±0.6~14.8±2.6μg·m L-1),且选择指数较高(SI>27)。结论本研究系统评价了11味常用中药提取物对LPS诱导RAW 264.7细胞产生NO的影响,研究结果进一步证实这些中药的抗炎活性。
Objective To determine the inhibitory effect of 11 traditional Chinese medicine （TCM） extracts on nitric oxide （NO） production in lipopolysaccharide （LPS） activated RAW 264.7 cells. Methods The cytotoxicity of the extracts was assessed by an MTT assay, and the NO production was determined by Griess method, in which N-monomethyl-L-arginine acetate （L-NMMA） was selected as the positive control. Results All 11 extracts significantly inhibited the NO release, and 5 of them possessed remarkable activities with IC5o at 4.3± 0.6 -- 14.8± 2.6 μg mL - J, and SI values higher than 〉 27. Conclusion This study further comfirms the anti-inflammatory effect of the 11 TCMs, and also provides references for the efficacy evaluation of anti-rheumatism herbs.
出处
《中南药学》
CAS
2018年第1期72-75,共4页
Central South Pharmacy
基金
国家自然科学基金(No.81603284)
陕西中医药大学重点培育项目(No.2016PY15)
2016~2017年度陕西省"春笋计划"课题研究项目
陕西省重点科技创新团队(No.2012KTC-20)
