一测多评法比较毛橘红与光橘红5种黄酮类成分含量

Comparison of content of five flavones in Citrus grandis “Tomentosa” and Citrus grandis with quantitative analysis of muli-components by single marker

目的建立一测多评法同时测定毛橘红与光橘红中5种黄酮类成分的含量,并进行分析。方法采用HPLC法,以柚皮苷为内参物,建立其与新橙皮苷、野漆树苷、柚皮素和芹菜素的相对校正因子,并利用相对校正因子计算毛橘红与光橘红样品中各成分含量。同时通过外标法计算各成分含量,并比较2种方法的差异。采用t检验比较分析毛橘红与光橘红中5种黄酮类成分含量。结果柚皮苷与新橙皮苷、野漆树苷、柚皮素、芹菜素的校正因子分别为0.898、1.519、0.313、0.406,RSD<3.0%。一测多评法与外标法计算得到的含量测定结果无显著差异。毛橘红与光橘红中的柚皮苷、野漆树苷、柚皮素含量存在显著差异。结论以柚皮苷为内参物建立的相对校正因子准确、可行,毛橘红与光橘红黄酮类成分存在很大差异,一测多评法可用于毛橘红与光橘红的质量控制。

Objective To establish quantitative analysis of multi-components by single marker （QAMS） to compare five flavones in Citrusgrandis＂Tomentosa＂andC. grandis. Methods Four relative correction factors （RCFs） of neohesperidin, rhoifolin, naringenin, apigenin were established in the HPLC method with the Naringin as internal standard, which were used to determine the content of four flavones in C. grandis ＂Tomentosa＂ and C. grandis. Meanwhile, external standard method （ESM） was employed to calculate the content of five flavones. The difference between ESM and QAMS were analyzed to evaluate the accuracy of QAMS. T-test was used to compare five flavones in C. grandis ＇Tomentosa＇ and C. grandis. Results RCFs of neohesperidin, rhoifolin, naringenin, apigenin were 0.898, 1.519, 0.313, 0.406, and repeatability was good in different experimental conditions （RSD 〈 3.0 %）. There were no significant differences in the quantitative analysis results of two methods. Content of naringin, rhoifolin and naringenin in two species were significant different. Conclusion The RCFs of neohesperidin, rhoifolin, naringenin, apigenin as reference to naringin are accurate and feasible. Content of flavones in Citrus grandis ＂Tomentosa＂ and C. grandis are obvious different. QASM can be applied as a strategy for quality control of C. grandis.