两种提取骨骼、牙齿DNA的方法

Two Methods for Extracting DNA from Ordinary and Aged Bones or Teeth

目的建立提取骨骼、牙齿DNA的新方法。方法收集380例骨骼、牙齿检材,其中347份为常规骨骼、牙齿(常规组),33份为陈旧骨骼、牙齿(疑难组)。常规组检材以Handy-Eco仪器、疑难组则用冷冻研磨仪研磨成粉,以Kingfisher自动化系统提取DNA,用Identifiler Plus进行STR分型检测。结果用HandyEco Kingfisher法(H-K法)成功提取345例常规骨骼、牙齿检材的DNA,成功率99.42%;用Freeze-mill Kingfisher法(FM-K法)成功获取32例疑难骨骼、牙齿检材的DNA,成功率96.97%。结论采用H-K法和FM-K法对骨骼和牙齿DNA的检验成功率较高,可选择应用于工作实践中。

Objective To set up new methods for the extraction of DNA from ordinary and aged bones or teeth. Methods 380 pieces of bone and tooth, 347 ordinary and 33 aged ones, were collected from the previous routine casework. Handy-Eco instrument was selected to grind the ordinary samples into powder while the aged by Freezer Mill apparatus to achieve the same effcacy. DNA was extracted from the above-processed samples with Kingfsher automatic system （thus the H-K and FM-K methods）, with the Identifler Plus kit to test the STR profle. Results DNA was shown of 15 autosomal STR loci of Identifler Plus from the 345 ordinary samples, presenting the extraction rate as 99.42%. 32 aged samples exposed their 15 autosomal STR loci of Identifler Plus, bringing up the DNA extraction rate about 96.97%. Conclusion The established methods here are effcient to extract DNA from either the ordinary or aged bones/teeth, applicable in forensic practice.